Fos expression following isotonic volume expansion of the unanesthetized male rat

R. R. Randolph, Q. Li, Kathleen Curtis, M. J. Sullivan, J. T. Cunningham

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Cardiopulmonary afferents, baroreceptor afferents, or atrial natriuretic peptide binding to circumventricular organs may mediate the central response to volume expansion, a condition common to pregnancy, exercise training, and congestive heart failure. This study used Fos immunocytochemistry to examine brain regions activated by volume expansion. Male Sprague-Dawley rats were infused with isotonic saline equal to 10% of their body weight in 10 min followed by a maintenance infusion of 0.5 ml/min for 110 min. Control animals received 2-h infusions at 0.01 ml/min. Five minutes after the start of volume expansion, central venous pressure of expanded animals was significantly greater than control animals. The volume-expanded group exhibited significantly greater Fos activation (P < 0.05) in the area postrema, nucleus of the solitary tract, caudal ventrolateral medulla, paraventricular nucleus, supraoptic nucleus, and perinuclear zone of the supraoptic nucleus. Double labeling indicates that oxytocinergic neurons in the supraoptic nucleus are activated. Neurons in brain regions known to inhibit both sympathetic activity and vasopressin release show increased Fos expression following isotonic volume expansion.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume274
Issue number5 43-5
StatePublished - 1 Jan 1998

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Supraoptic Nucleus
Area Postrema
Neurons
Pressoreceptors
Central Venous Pressure
Solitary Nucleus
Paraventricular Hypothalamic Nucleus
Brain
Atrial Natriuretic Factor
Vasopressins
Sprague Dawley Rats
Heart Failure
Immunohistochemistry
Body Weight
Maintenance
Exercise
Pregnancy

Keywords

  • Atrial natriuretic peptide
  • Cardiopulmonary receptors
  • Oxytocin
  • Vasopressin

Cite this

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abstract = "Cardiopulmonary afferents, baroreceptor afferents, or atrial natriuretic peptide binding to circumventricular organs may mediate the central response to volume expansion, a condition common to pregnancy, exercise training, and congestive heart failure. This study used Fos immunocytochemistry to examine brain regions activated by volume expansion. Male Sprague-Dawley rats were infused with isotonic saline equal to 10{\%} of their body weight in 10 min followed by a maintenance infusion of 0.5 ml/min for 110 min. Control animals received 2-h infusions at 0.01 ml/min. Five minutes after the start of volume expansion, central venous pressure of expanded animals was significantly greater than control animals. The volume-expanded group exhibited significantly greater Fos activation (P < 0.05) in the area postrema, nucleus of the solitary tract, caudal ventrolateral medulla, paraventricular nucleus, supraoptic nucleus, and perinuclear zone of the supraoptic nucleus. Double labeling indicates that oxytocinergic neurons in the supraoptic nucleus are activated. Neurons in brain regions known to inhibit both sympathetic activity and vasopressin release show increased Fos expression following isotonic volume expansion.",
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Fos expression following isotonic volume expansion of the unanesthetized male rat. / Randolph, R. R.; Li, Q.; Curtis, Kathleen; Sullivan, M. J.; Cunningham, J. T.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 274, No. 5 43-5, 01.01.1998.

Research output: Contribution to journalArticle

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