TY - JOUR
T1 - Fodinicurvata halophila sp. nov., a moderately halophilic bacterium from a marine saltern
AU - Infante-Dominguez, Carmen
AU - Lawson, Paul A.
AU - Johnson, Crystal N.
AU - Sánchez-Porro, Cristina
AU - Ventosa, Antonio
N1 - Publisher Copyright:
© 2015 IUMS.
PY - 2015
Y1 - 2015
N2 - A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45ALT, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase-and oxidase-positive. Strain BA45ALT grew at temperatures in the range 14-45 °C (optimally at 37 °C), at pH 5.0-9.0 (optimally at pH 7.5), and in media containing 5-20% (w/v) salts [optimally in media containing 10% (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45ALT is a member of the genus Fodinicurvata. The closest relatives to the novel strain were Fodinicurvata fenggangensis YIM D812T and Fodinicurvata sediminis YIM D82T with sequence similarities of 98.2% and 97.4%, respectively. DNA-DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30% and 15%, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45ALT were C18: 1ɷ7c, C16: 0 and iso-C15: 0. The G+C content of the genomic DNA of strain BA45ALT was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45ALT constituted a novel species of the genus Fodinicurvata, for which the name Fodinicurvata halophila sp. nov. is suggested. The type strain is BA45ALT (=CCM 8504T=CECT 8472T=JCM 19075T=LMG 27945T).
AB - A Gram-stain-negative, rod-shaped, facultatively anaerobic, moderately halophilic bacterium, designated strain BA45ALT, was isolated from water of a saltern located in Santa Pola, Alicante, Spain. Cells were motile, and catalase-and oxidase-positive. Strain BA45ALT grew at temperatures in the range 14-45 °C (optimally at 37 °C), at pH 5.0-9.0 (optimally at pH 7.5), and in media containing 5-20% (w/v) salts [optimally in media containing 10% (w/v) salts]. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA45ALT is a member of the genus Fodinicurvata. The closest relatives to the novel strain were Fodinicurvata fenggangensis YIM D812T and Fodinicurvata sediminis YIM D82T with sequence similarities of 98.2% and 97.4%, respectively. DNA-DNA hybridization between the novel isolate and these phylogenetically related species revealed relatedness values of 30% and 15%, respectively, with respect to the aforementioned species. The major cellular fatty acids of strain BA45ALT were C18: 1ɷ7c, C16: 0 and iso-C15: 0. The G+C content of the genomic DNA of strain BA45ALT was 58.0 mol%, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine and a number of unknown phospholipids and lipids. Based on phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA45ALT constituted a novel species of the genus Fodinicurvata, for which the name Fodinicurvata halophila sp. nov. is suggested. The type strain is BA45ALT (=CCM 8504T=CECT 8472T=JCM 19075T=LMG 27945T).
UR - http://www.scopus.com/inward/record.url?scp=84925296338&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.000010
DO - 10.1099/ijs.0.000010
M3 - Article
C2 - 25479951
AN - SCOPUS:84925296338
SN - 1466-5026
VL - 65
SP - 766
EP - 771
JO - International journal of systematic and evolutionary microbiology
JF - International journal of systematic and evolutionary microbiology
IS - 3
ER -