Fine structure and immunofluorescent studies of the wish cell line

William D. Meek, Walter L. Davis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Morphological studies of the WISH cell line reveal an epithelioid cell type with some characteristics of both the original human amnion epithelium and a transformed state. WISH cells have a cytoplasm filled with microtubules; however, actin filament bundles are few, with actin localized at areas of cell contact and arranged diffusely through the cytoplasm, as viewed by indirect immunofluorescence. Fingerlike projections or short filopodia are observed connecting cells that grow in a closely apposed monolayer. Other surface features, as viewed by scanning electron microscopy, include microvilli and blebs. Transmission electron microscopy shows that WISH cultures consist of light or dark cells with organelles that include lipid droplets, abundant free ribosomes, tubular mitochondria, lysosomes, annulate lamellae, rough endoplasmic reticulum, 6-nm microfilaments, 10-nm intermediate filaments, and microtubules. Pleomorphic nuclei with multiple nucleoli and fibrillar nuclear bodies are common. Desmosomes and subsurface confronting cisternae connect cells. To our knowledge, these structural studies are the first to describe WISH and lead to subsequent investigation of the cell surface phenomenon of blebbing and surface charge in WISH and another human cell line.

Original languageEnglish
Pages (from-to)716-724
Number of pages9
JournalIn Vitro Cellular & Developmental Biology
Volume22
Issue number12
DOIs
StatePublished - 1 Dec 1986
Externally publishedYes

Fingerprint

Cells
Cell Line
Actins
Surface phenomena
Mitochondria
Blister
Surface charge
Actin Cytoskeleton
Microtubules
Monolayers
Cytoplasm
Transmission electron microscopy
Lipids
Epithelioid Cells
Desmosomes
Scanning electron microscopy
Pseudopodia
Amnion
Intermediate Filaments
Rough Endoplasmic Reticulum

Keywords

  • actin
  • amnion
  • immunofluorescence
  • tubulin
  • ultrastructure
  • WISH

Cite this

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Fine structure and immunofluorescent studies of the wish cell line. / Meek, William D.; Davis, Walter L.

In: In Vitro Cellular & Developmental Biology, Vol. 22, No. 12, 01.12.1986, p. 716-724.

Research output: Contribution to journalArticle

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