Estrogen regulation of lactoferrin expression in human endometrium

Mae Ellen Kelver, Anil Kaul, Bogdan Nowicki, William E. Findley, T. William Hutchens, Manubai Nagamani

Research output: Contribution to journalArticle

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Abstract

PROBLEM: Lactoferrin is an iron-binding glycoprotein that has been shown to be overexpressed in human endometrial carcinomas. The purpose of our present study is to investigate the possible role of estradiol in the expression of lactoferrin. METHOD OF STUDY: We investigated 1) serum levels of lactoferrin in five women during normal ovulatory cycles, 2) serum levels of lactoferrin during ten human menopausal gonadotropin induced cycles when estradiol levels are high, and 3) lactoferrin expression in five proliferative and five secretory phase endometrium by immunohistochemical studies. The serum concentrations of lactoferrin were measured by a peroxidase-based enzyme-linked immunosorbent assay. RESULTS: In normal ovulatory cycles, the mean serum lactoferrin concentration during the proliferative phase (0.4013 ± 0.0242 μg/mL) was significantly higher (P<0.02) than in the secretory phase (0.3468 ± 0.0209 μg/mL). In induced cycles, there was gradual increase in lactoferrin levels with increasing estradiol concentrations. Peak lactoferrin levels in induced cycles (0.7495 ± 0.1148 μg/mL) were significantly higher (P<0.003) than the midcycle levels (0.423 ± 0.0424 μg/mL) in normal cycles. Immunohistochemical analysis of the endometrium revealed greater expression of lactoferrin in proliferative endometrium (50.7 ± 13%, range 28-72%) than in secretory endometrium (19.2 ± 4%, range 7-31%). CONCLUSION: These results indicate that estradiol may play a role in the regulation of lactoferrin expression in human endometrium.

Original languageEnglish
Pages (from-to)243-247
Number of pages5
JournalAmerican Journal of Reproductive Immunology
Volume36
Issue number5
DOIs
StatePublished - 1 Jan 1996

Fingerprint

Lactoferrin
Endometrium
Estrogens
Estradiol
Serum
Menotropins
Endometrial Neoplasms
Peroxidase
Glycoproteins
Iron
Enzyme-Linked Immunosorbent Assay

Keywords

  • Estradiol
  • Lactotransferrin
  • Uterus

Cite this

Kelver, Mae Ellen ; Kaul, Anil ; Nowicki, Bogdan ; Findley, William E. ; Hutchens, T. William ; Nagamani, Manubai. / Estrogen regulation of lactoferrin expression in human endometrium. In: American Journal of Reproductive Immunology. 1996 ; Vol. 36, No. 5. pp. 243-247.
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abstract = "PROBLEM: Lactoferrin is an iron-binding glycoprotein that has been shown to be overexpressed in human endometrial carcinomas. The purpose of our present study is to investigate the possible role of estradiol in the expression of lactoferrin. METHOD OF STUDY: We investigated 1) serum levels of lactoferrin in five women during normal ovulatory cycles, 2) serum levels of lactoferrin during ten human menopausal gonadotropin induced cycles when estradiol levels are high, and 3) lactoferrin expression in five proliferative and five secretory phase endometrium by immunohistochemical studies. The serum concentrations of lactoferrin were measured by a peroxidase-based enzyme-linked immunosorbent assay. RESULTS: In normal ovulatory cycles, the mean serum lactoferrin concentration during the proliferative phase (0.4013 ± 0.0242 μg/mL) was significantly higher (P<0.02) than in the secretory phase (0.3468 ± 0.0209 μg/mL). In induced cycles, there was gradual increase in lactoferrin levels with increasing estradiol concentrations. Peak lactoferrin levels in induced cycles (0.7495 ± 0.1148 μg/mL) were significantly higher (P<0.003) than the midcycle levels (0.423 ± 0.0424 μg/mL) in normal cycles. Immunohistochemical analysis of the endometrium revealed greater expression of lactoferrin in proliferative endometrium (50.7 ± 13{\%}, range 28-72{\%}) than in secretory endometrium (19.2 ± 4{\%}, range 7-31{\%}). CONCLUSION: These results indicate that estradiol may play a role in the regulation of lactoferrin expression in human endometrium.",
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Estrogen regulation of lactoferrin expression in human endometrium. / Kelver, Mae Ellen; Kaul, Anil; Nowicki, Bogdan; Findley, William E.; Hutchens, T. William; Nagamani, Manubai.

In: American Journal of Reproductive Immunology, Vol. 36, No. 5, 01.01.1996, p. 243-247.

Research output: Contribution to journalArticle

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T1 - Estrogen regulation of lactoferrin expression in human endometrium

AU - Kelver, Mae Ellen

AU - Kaul, Anil

AU - Nowicki, Bogdan

AU - Findley, William E.

AU - Hutchens, T. William

AU - Nagamani, Manubai

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