Effects of sub-minimum inhibitory concentration antibiotic levels and temperature on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Heamophilus somnus

Brenda Y. Reeks, Franklin R. Champlin, Daniel B. Paulsen, Daniel W. Scruggs, Mark L. Lawrence

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The objective of this study was to determine the effects of sub-minimum inhibitory concentrations (sub-MICs) of 2 veterinary antibiotic preparations, chlortetracycline (CTC) and chlortetracycline-sulfamethazine (CTC + SMZ), on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus at normal and febrile body temperatures. Sub-minimum inhibitory concentrations of both antibiotics reduced the growth rates of M. haemolytica and H. somnus. Growth of both species was not inhibited when grown at 41°C compared to 37°C. There was no detectable consistent effect of antibiotic or temperature on outer membrane protein expression for either species. Our study indicates that sub-MIC levels of CTC and CTC + SMZ markedly impair growth of clinical M. haemolytica and H. somnus isolates, potentially allowing more effective host clearance during infection.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalCanadian Journal of Veterinary Research
Volume69
Issue number1
Publication statusPublished - 1 Jan 2005

    Fingerprint

Cite this