Effects of sub-minimum inhibitory concentration antibiotic levels and temperature on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Heamophilus somnus

Brenda Y. Reeks, Franklin Champlin, Daniel B. Paulsen, Daniel W. Scruggs, Mark L. Lawrence

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The objective of this study was to determine the effects of sub-minimum inhibitory concentrations (sub-MICs) of 2 veterinary antibiotic preparations, chlortetracycline (CTC) and chlortetracycline-sulfamethazine (CTC + SMZ), on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus at normal and febrile body temperatures. Sub-minimum inhibitory concentrations of both antibiotics reduced the growth rates of M. haemolytica and H. somnus. Growth of both species was not inhibited when grown at 41°C compared to 37°C. There was no detectable consistent effect of antibiotic or temperature on outer membrane protein expression for either species. Our study indicates that sub-MIC levels of CTC and CTC + SMZ markedly impair growth of clinical M. haemolytica and H. somnus isolates, potentially allowing more effective host clearance during infection.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalCanadian Journal of Veterinary Research
Volume69
Issue number1
StatePublished - 1 Jan 2005

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Mannheimia haemolytica
Chlortetracycline
Haemophilus somnus
chlortetracycline
outer membrane proteins
Microbial Sensitivity Tests
minimum inhibitory concentration
Membrane Proteins
protein synthesis
antibiotics
Anti-Bacterial Agents
kinetics
Temperature
Growth
temperature
Body Temperature
Fever
body temperature
fever
Infection

Cite this

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Effects of sub-minimum inhibitory concentration antibiotic levels and temperature on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Heamophilus somnus. / Reeks, Brenda Y.; Champlin, Franklin; Paulsen, Daniel B.; Scruggs, Daniel W.; Lawrence, Mark L.

In: Canadian Journal of Veterinary Research, Vol. 69, No. 1, 01.01.2005, p. 1-10.

Research output: Contribution to journalArticle

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