Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration

Thomas R. Shryock, Scott A. Silver, Martin W. Banschbach, John C. Kramer

Research output: Contribution to journalArticle

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Abstract

The effect of Pseudomonas aeruginosa heat-stable hemolysin (rhamnolipid) on human neutrophil migration has been investigated. Rhamnolipid was prepared from culture filtrate and characterized by thin-layer chromatography. The lytic activity of rhamnolipid was quantitated by titration against neutrophils. Leukocyte migration response was measured using51Cr-labeled neutrophils with a double-filter technique in modified Boyden chambers. The results suggest rhamnolipid stimulated chemotaxis as well as chemokinesis. Moreover, rhamnolipid impaired a chemotactic response to N-formyl-methionyl-leucyl-phenylalanine. These effects may be important in host-parasite interactions.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalCurrent Microbiology
Volume10
Issue number6
DOIs
StatePublished - 1 Nov 1984

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Pseudomonas aeruginosa
Neutrophils
N-Formylmethionine Leucyl-Phenylalanine
Host-Parasite Interactions
Hemolysin Proteins
Chemotaxis
Thin Layer Chromatography
Leukocytes
Hot Temperature
rhamnolipid

Cite this

Shryock, Thomas R. ; Silver, Scott A. ; Banschbach, Martin W. ; Kramer, John C. / Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration. In: Current Microbiology. 1984 ; Vol. 10, No. 6. pp. 323-328.
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Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration. / Shryock, Thomas R.; Silver, Scott A.; Banschbach, Martin W.; Kramer, John C.

In: Current Microbiology, Vol. 10, No. 6, 01.11.1984, p. 323-328.

Research output: Contribution to journalArticle

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