Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration

Thomas R. Shryock, Scott A. Silver, Martin W. Banschbach, John C. Kramer

Research output: Contribution to journalArticle

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Abstract

The effect of Pseudomonas aeruginosa heat-stable hemolysin (rhamnolipid) on human neutrophil migration has been investigated. Rhamnolipid was prepared from culture filtrate and characterized by thin-layer chromatography. The lytic activity of rhamnolipid was quantitated by titration against neutrophils. Leukocyte migration response was measured using51Cr-labeled neutrophils with a double-filter technique in modified Boyden chambers. The results suggest rhamnolipid stimulated chemotaxis as well as chemokinesis. Moreover, rhamnolipid impaired a chemotactic response to N-formyl-methionyl-leucyl-phenylalanine. These effects may be important in host-parasite interactions.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalCurrent Microbiology
Volume10
Issue number6
DOIs
Publication statusPublished - 1 Nov 1984

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