Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration

Thomas R. Shryock; Scott A. Silver; Martin W. Banschbach; John C. Kramer

doi:10.1007/BF01626559

Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration

Thomas R. Shryock, Scott A. Silver, Martin W. Banschbach, John C. Kramer

Biochemistry & Microbiology

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Abstract

The effect of Pseudomonas aeruginosa heat-stable hemolysin (rhamnolipid) on human neutrophil migration has been investigated. Rhamnolipid was prepared from culture filtrate and characterized by thin-layer chromatography. The lytic activity of rhamnolipid was quantitated by titration against neutrophils. Leukocyte migration response was measured using⁵¹Cr-labeled neutrophils with a double-filter technique in modified Boyden chambers. The results suggest rhamnolipid stimulated chemotaxis as well as chemokinesis. Moreover, rhamnolipid impaired a chemotactic response to N-formyl-methionyl-leucyl-phenylalanine. These effects may be important in host-parasite interactions.

Original language	English
Pages (from-to)	323-328
Number of pages	6
Journal	Current Microbiology
Volume	10
Issue number	6
DOIs	https://doi.org/10.1007/BF01626559
State	Published - Nov 1984

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title = "Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration",

abstract = "The effect of Pseudomonas aeruginosa heat-stable hemolysin (rhamnolipid) on human neutrophil migration has been investigated. Rhamnolipid was prepared from culture filtrate and characterized by thin-layer chromatography. The lytic activity of rhamnolipid was quantitated by titration against neutrophils. Leukocyte migration response was measured using51Cr-labeled neutrophils with a double-filter technique in modified Boyden chambers. The results suggest rhamnolipid stimulated chemotaxis as well as chemokinesis. Moreover, rhamnolipid impaired a chemotactic response to N-formyl-methionyl-leucyl-phenylalanine. These effects may be important in host-parasite interactions.",

author = "Shryock, {Thomas R.} and Silver, {Scott A.} and Banschbach, {Martin W.} and Kramer, {John C.}",

year = "1984",

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AU - Shryock, Thomas R.

AU - Silver, Scott A.

AU - Banschbach, Martin W.

AU - Kramer, John C.

PY - 1984/11

Y1 - 1984/11

N2 - The effect of Pseudomonas aeruginosa heat-stable hemolysin (rhamnolipid) on human neutrophil migration has been investigated. Rhamnolipid was prepared from culture filtrate and characterized by thin-layer chromatography. The lytic activity of rhamnolipid was quantitated by titration against neutrophils. Leukocyte migration response was measured using51Cr-labeled neutrophils with a double-filter technique in modified Boyden chambers. The results suggest rhamnolipid stimulated chemotaxis as well as chemokinesis. Moreover, rhamnolipid impaired a chemotactic response to N-formyl-methionyl-leucyl-phenylalanine. These effects may be important in host-parasite interactions.

AB - The effect of Pseudomonas aeruginosa heat-stable hemolysin (rhamnolipid) on human neutrophil migration has been investigated. Rhamnolipid was prepared from culture filtrate and characterized by thin-layer chromatography. The lytic activity of rhamnolipid was quantitated by titration against neutrophils. Leukocyte migration response was measured using51Cr-labeled neutrophils with a double-filter technique in modified Boyden chambers. The results suggest rhamnolipid stimulated chemotaxis as well as chemokinesis. Moreover, rhamnolipid impaired a chemotactic response to N-formyl-methionyl-leucyl-phenylalanine. These effects may be important in host-parasite interactions.

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VL - 10

SP - 323

EP - 328

JO - Current Microbiology

JF - Current Microbiology

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ER -

Effect of Pseudomonas aeruginosa rhamnolipid on human neutrophil migration

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