Effect of pap copy number and receptor specificity on virulence of fimbriated Escherichia coli in a murine urinary tract colonization model

Richard A. Hull; Bogdan Nowicki; Anil Kaul; Rebecca Runyan; Catharina Svanborg; Sheila I. Hull

doi:10.1006/mpat.1994.1054

Effect of pap copy number and receptor specificity on virulence of fimbriated Escherichia coli in a murine urinary tract colonization model

Richard A. Hull, Bogdan Nowicki, Anil Kaul, Rebecca Runyan, Catharina Svanborg, Sheila I. Hull

School of Health Care Administration

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Escherichia coli FN506 containing pap genes that encode two different P fimbriae adherence specificity types were tested for virulence in a murine urinary colonization model. Strains containing adherence genes on either high copy or low copy plasmids were compared. Bacteria that harbored the adherence genes on high copy plasmids colonized mouse kidneys less well than bacteria with the same adherence genes in low copy even though the high copy strains exhibited greater hemagglutination capacity. Bacteria with either type of P fimbriae were able to colonize but pap-2⁺ bacteria showed increased colonizing capacity when strains containing pap-1 or pap -2 genes on low copy plasmids were compared. Bacteria containing plasmids with both adherence specificities had a similar colonizing capacity as bacteria with either type separately.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	Microbial Pathogenesis
Volume	17
Issue number	2
DOIs	https://doi.org/10.1006/mpat.1994.1054
State	Published - Aug 1994

Keywords

Adherence
Colonization
Escherichia coli
Murine
P fimbriae
Pap gene

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10.1006/mpat.1994.1054

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title = "Effect of pap copy number and receptor specificity on virulence of fimbriated Escherichia coli in a murine urinary tract colonization model",

abstract = "Escherichia coli FN506 containing pap genes that encode two different P fimbriae adherence specificity types were tested for virulence in a murine urinary colonization model. Strains containing adherence genes on either high copy or low copy plasmids were compared. Bacteria that harbored the adherence genes on high copy plasmids colonized mouse kidneys less well than bacteria with the same adherence genes in low copy even though the high copy strains exhibited greater hemagglutination capacity. Bacteria with either type of P fimbriae were able to colonize but pap-2+ bacteria showed increased colonizing capacity when strains containing pap-1 or pap -2 genes on low copy plasmids were compared. Bacteria containing plasmids with both adherence specificities had a similar colonizing capacity as bacteria with either type separately.",

keywords = "Adherence, Colonization, Escherichia coli, Murine, P fimbriae, Pap gene",

author = "Hull, {Richard A.} and Bogdan Nowicki and Anil Kaul and Rebecca Runyan and Catharina Svanborg and Hull, {Sheila I.}",

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T1 - Effect of pap copy number and receptor specificity on virulence of fimbriated Escherichia coli in a murine urinary tract colonization model

AU - Hull, Richard A.

AU - Nowicki, Bogdan

AU - Kaul, Anil

AU - Runyan, Rebecca

AU - Svanborg, Catharina

AU - Hull, Sheila I.

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AB - Escherichia coli FN506 containing pap genes that encode two different P fimbriae adherence specificity types were tested for virulence in a murine urinary colonization model. Strains containing adherence genes on either high copy or low copy plasmids were compared. Bacteria that harbored the adherence genes on high copy plasmids colonized mouse kidneys less well than bacteria with the same adherence genes in low copy even though the high copy strains exhibited greater hemagglutination capacity. Bacteria with either type of P fimbriae were able to colonize but pap-2+ bacteria showed increased colonizing capacity when strains containing pap-1 or pap -2 genes on low copy plasmids were compared. Bacteria containing plasmids with both adherence specificities had a similar colonizing capacity as bacteria with either type separately.

KW - Adherence

KW - Colonization

KW - Escherichia coli

KW - Murine

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KW - Pap gene

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Effect of pap copy number and receptor specificity on virulence of fimbriated Escherichia coli in a murine urinary tract colonization model

Abstract

Keywords

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