Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: Evidence for opioid-immune crosstalk

Shekher Mohan; Randall L. Davis; Udaya DeSilva; Craig W. Stevens

doi:10.1016/j.jneuroim.2010.06.007

Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: Evidence for opioid-immune crosstalk

Shekher Mohan, Randall L. Davis, Udaya DeSilva, Craig W. Stevens

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Treatment of SK-N-SH cells with morphine and interleukin-1. beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

Original language	English
Pages (from-to)	26-34
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	227
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2010.06.007
State	Published - Oct 2010

Keywords

Interleukin-1β
Morphine
Mu opioid receptor
Opioid-immune crosstalk
SK-N-SH cells

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10.1016/j.jneuroim.2010.06.007

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@article{8520eba6363449b08f9db4be90543702,

title = "Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: Evidence for opioid-immune crosstalk",

abstract = "Treatment of SK-N-SH cells with morphine and interleukin-1. beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.",

keywords = "Interleukin-1β, Morphine, Mu opioid receptor, Opioid-immune crosstalk, SK-N-SH cells",

author = "Shekher Mohan and Davis, \{Randall L.\} and Udaya DeSilva and Stevens, \{Craig W.\}",

note = "Funding Information: This work was supported in part by NIH grants NS 062664 (RLD) and DA 012448 (CWS). We are grateful to Dr. S. C. Fernando, Ph.D. and Daniel Buck for their technical assistance.",

year = "2010",

month = oct,

doi = "10.1016/j.jneuroim.2010.06.007",

language = "English",

volume = "227",

pages = "26--34",

journal = "Journal of Neuroimmunology",

issn = "0165-5728",

publisher = "Elsevier B.V.",

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}

TY - JOUR

T1 - Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β

T2 - Evidence for opioid-immune crosstalk

AU - Mohan, Shekher

AU - Davis, Randall L.

AU - DeSilva, Udaya

AU - Stevens, Craig W.

N1 - Funding Information: This work was supported in part by NIH grants NS 062664 (RLD) and DA 012448 (CWS). We are grateful to Dr. S. C. Fernando, Ph.D. and Daniel Buck for their technical assistance.

PY - 2010/10

Y1 - 2010/10

N2 - Treatment of SK-N-SH cells with morphine and interleukin-1. beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

AB - Treatment of SK-N-SH cells with morphine and interleukin-1. beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

KW - Interleukin-1β

KW - Morphine

KW - Mu opioid receptor

KW - Opioid-immune crosstalk

KW - SK-N-SH cells

UR - https://www.scopus.com/pages/publications/77956651049

U2 - 10.1016/j.jneuroim.2010.06.007

DO - 10.1016/j.jneuroim.2010.06.007

M3 - Article

C2 - 20615556

AN - SCOPUS:77956651049

SN - 0165-5728

VL - 227

SP - 26

EP - 34

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: Evidence for opioid-immune crosstalk

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Keywords

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