Abstract
Treatment of SK-N-SH cells with morphine and interleukin-1. beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.
Original language | English |
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Pages (from-to) | 26-34 |
Number of pages | 9 |
Journal | Journal of Neuroimmunology |
Volume | 227 |
Issue number | 1-2 |
DOIs | |
State | Published - Oct 2010 |
Keywords
- Interleukin-1β
- Morphine
- Mu opioid receptor
- Opioid-immune crosstalk
- SK-N-SH cells