Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: Evidence for opioid-immune crosstalk

Shekher Mohan, Randall Davis, Udaya DeSilva, Craig Stevens

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Treatment of SK-N-SH cells with morphine and interleukin-1. beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

Original languageEnglish
Pages (from-to)26-34
Number of pages9
JournalJournal of Neuroimmunology
Volume227
Issue number1-2
DOIs
StatePublished - 1 Oct 2010

Fingerprint

mu Opioid Receptor
Neuroblastoma
Interleukin-1
Morphine
Opioid Analgesics
Interleukin-1 Type I Receptors
Messenger RNA
Naltrexone
Interleukin-1beta
Real-Time Polymerase Chain Reaction
Up-Regulation
Down-Regulation
Proteins

Keywords

  • Interleukin-1β
  • Morphine
  • Mu opioid receptor
  • Opioid-immune crosstalk
  • SK-N-SH cells

Cite this

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abstract = "Treatment of SK-N-SH cells with morphine and interleukin-1. beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.",
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Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β : Evidence for opioid-immune crosstalk. / Mohan, Shekher; Davis, Randall; DeSilva, Udaya; Stevens, Craig.

In: Journal of Neuroimmunology, Vol. 227, No. 1-2, 01.10.2010, p. 26-34.

Research output: Contribution to journalArticle

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AU - Davis, Randall

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