Dramatic saccharide-mediated protection of chaotropic-induced deactivation of concanavalin A

Débora N. Figlas, Hugo R. Arias, Ariel Fernández, Daniel M. Alperin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This work provides evidence of a physical instance in which some proteins that are usually inactivated under strong chaotropic conditions may become fully resistant through the occupancy of their binding sites with suitable ligands. In this regard, we found that Moluccella laevis lectin remains stable in the presence of denaturant concentrations of urea when an appropriate saccharide is bound to the protein (Alperin, D. M., Latter, H., Lis, H., and Sharon, N. (1992) Biochem. J. 285, 1-4). Extending this finding, we now demonstrate that the occupancy of the ligand binding sites of concanavalin A (Con A) with appropriate carbohydrates completely prevents the denaturation course elicited by 8 M urea at pH 7.4. In addition, the protecting efficiency of the saccharides was shown to be directly related to their specificities for the lectin. The observed saccharide protection follows the order: methyl α-D-mannopyranoside > methyl α-D-glucopyranoside > mannose > fructose > glucose. Concomitantly, the active tetrameric lectin with a molecular mass of ~105 kDa is preserved in 8 M urea when methyl α- D-mannopyranoside (100 mM) is present in the medium.

Original languageEnglish
Pages (from-to)154-158
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume340
Issue number1
DOIs
StatePublished - 1 Apr 1997
Externally publishedYes

Fingerprint

Concanavalin A
Lectins
Urea
Concanavalin A Receptors
Ligands
Denaturation
Molecular mass
Mannose
Fructose
Proteins
Binding Sites
Carbohydrates
Glucose
methylmannoside

Keywords

  • chaotropic effect
  • chaotropic effect suppression
  • concanavalin A
  • lectin
  • protein unfolding
  • protein unfolding protection

Cite this

Figlas, Débora N. ; Arias, Hugo R. ; Fernández, Ariel ; Alperin, Daniel M. / Dramatic saccharide-mediated protection of chaotropic-induced deactivation of concanavalin A. In: Archives of Biochemistry and Biophysics. 1997 ; Vol. 340, No. 1. pp. 154-158.
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Dramatic saccharide-mediated protection of chaotropic-induced deactivation of concanavalin A. / Figlas, Débora N.; Arias, Hugo R.; Fernández, Ariel; Alperin, Daniel M.

In: Archives of Biochemistry and Biophysics, Vol. 340, No. 1, 01.04.1997, p. 154-158.

Research output: Contribution to journalArticle

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