Divergent effects of ERα and ERβ on fluid intake by female rats are not dependent on concomitant changes in AT1R expression or body weight

Jessica Santollo, Anikó Marshall, Kathleen Curtis, Robert C. Speth, Stewart D. Clark, Derek Daniels

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Estradiol (E2) decreases both water and saline intakes by female rats. The ERα and ERβ subtypes are expressed in areas of the brain that control fluid intake; however, the role that these receptors play in E2’s antidipsogenic and antinatriorexigenic effects have not been examined. Accordingly, we tested the hypothesis that activation of ERα and ERβ decreases water and saline intakes by female rats. We found a divergence in E2’s inhibitory effect on intake: activation of ERα decreased water intake, whereas activation of ERβ decreased saline intake. E2 decreases expression of the angiotensin II type 1 receptor (AT1R), a receptor with known relevance to water and salt intakes, in multiple areas of the brain where ERα and ERβ are differentially expressed. Therefore, we tested for agonist-induced changes in AT1R mRNA expression by RT-PCR and protein expression by analyzing receptor binding to test the hypothesis that the divergent effects of these ER subtypes are mediated by region-specific changes in AT1R expression. Although we found no changes in AT1R mRNA or binding in areas of the brain known to control fluid intake associated with agonist treatment, the experimental results replicate and extend previous findings that body weight changes mediate alterations in AT1R expression in distinct brain regions. Together, the results reveal selective effects of ER subtypes on ingestive behaviors, advancing our understanding of E2’s inhibitory role in the controls of fluid intake by female rats.

Original languageEnglish
Pages (from-to)R14-R23
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume311
Issue number1
DOIs
StatePublished - 1 Jul 2016

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Angiotensin Type 1 Receptor
Body Weight
Drinking
Brain
Messenger RNA
Body Weight Changes
Estradiol
Salts
Polymerase Chain Reaction
Proteins

Keywords

  • Angiotensin II type 1 receptor
  • Brain
  • Drinking microstructure
  • Estrogen
  • Estrogen receptors
  • Fluid balance
  • Water intake

Cite this

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title = "Divergent effects of ERα and ERβ on fluid intake by female rats are not dependent on concomitant changes in AT1R expression or body weight",
abstract = "Estradiol (E2) decreases both water and saline intakes by female rats. The ERα and ERβ subtypes are expressed in areas of the brain that control fluid intake; however, the role that these receptors play in E2’s antidipsogenic and antinatriorexigenic effects have not been examined. Accordingly, we tested the hypothesis that activation of ERα and ERβ decreases water and saline intakes by female rats. We found a divergence in E2’s inhibitory effect on intake: activation of ERα decreased water intake, whereas activation of ERβ decreased saline intake. E2 decreases expression of the angiotensin II type 1 receptor (AT1R), a receptor with known relevance to water and salt intakes, in multiple areas of the brain where ERα and ERβ are differentially expressed. Therefore, we tested for agonist-induced changes in AT1R mRNA expression by RT-PCR and protein expression by analyzing receptor binding to test the hypothesis that the divergent effects of these ER subtypes are mediated by region-specific changes in AT1R expression. Although we found no changes in AT1R mRNA or binding in areas of the brain known to control fluid intake associated with agonist treatment, the experimental results replicate and extend previous findings that body weight changes mediate alterations in AT1R expression in distinct brain regions. Together, the results reveal selective effects of ER subtypes on ingestive behaviors, advancing our understanding of E2’s inhibitory role in the controls of fluid intake by female rats.",
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Divergent effects of ERα and ERβ on fluid intake by female rats are not dependent on concomitant changes in AT1R expression or body weight. / Santollo, Jessica; Marshall, Anikó; Curtis, Kathleen; Speth, Robert C.; Clark, Stewart D.; Daniels, Derek.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 311, No. 1, 01.07.2016, p. R14-R23.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Divergent effects of ERα and ERβ on fluid intake by female rats are not dependent on concomitant changes in AT1R expression or body weight

AU - Santollo, Jessica

AU - Marshall, Anikó

AU - Curtis, Kathleen

AU - Speth, Robert C.

AU - Clark, Stewart D.

AU - Daniels, Derek

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