Abstract
This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C. perfringens.
Original language | English |
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Pages (from-to) | 233-240 |
Number of pages | 8 |
Journal | FEMS Microbiology Letters |
Volume | 233 |
Issue number | 2 |
DOIs | |
State | Published - 15 Apr 2004 |
Externally published | Yes |
Keywords
- C. perfringens
- Enterotoxin
- spo0A
- Sporulation