Disruption of the gene (spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A

I. Hsiu Huang, Michael Waters, Roberto R. Grau, Mahfuzur R. Sarker

Research output: Contribution to journalArticle

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This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C. perfringens.

Original languageEnglish
Pages (from-to)233-240
Number of pages8
JournalFEMS Microbiology Letters
Issue number2
StatePublished - 15 Apr 2004
Externally publishedYes



  • C. perfringens
  • Enterotoxin
  • spo0A
  • Sporulation

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