@article{238352174a6a4630a93db2717565269f,
title = "Disruption of the gene (spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A",
abstract = "This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C. perfringens.",
keywords = "C. perfringens, Enterotoxin, spo0A, Sporulation",
author = "Huang, {I. Hsiu} and Michael Waters and Grau, {Roberto R.} and Sarker, {Mahfuzur R.}",
note = "Funding Information: This research was supported by a grant from the N.L. Tartar Foundation of Oregon State University, by a grant from Medical Research Foundation of Oregon Health Science University, by a grant from Agricultural Research Foundation of Oregon State University and by USDA Grant 2002-02281 from the Ensuring Food Safety Research Program (all to M.R.S.). We are grateful to Dr. B.A. McClane, University of Pittsburgh School of Medicine, for providing us with CPE antibody. We thank Nahid Mahfuz for her technical assistance and Dr. D.D. Rockey for his critical editorial comments.",
year = "2004",
month = apr,
day = "15",
doi = "10.1016/j.femsle.2004.02.014",
language = "English",
volume = "233",
pages = "233--240",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "2",
}