Discovery, characterization, and remediation of a C-terminal Fc-extension in proteins expressed in CHO cells

Keyphrases

• Chinese Hamster Ovary Cells 100%
• Impurities 100%
• Glycine 66%
• Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) 66%
• C-terminus 66%
• Product Quality 66%
• Codon 66%
• Protein-coding Genes 33%
• Post-translational Modification 33%
• Amino Acid Sequence 33%
• Promoter Region 33%
• Mass Spectrometry 33%
• Therapeutic Proteins 33%
• Protein-based 33%
• Cassette 33%
• Splicing Event 33%
• Vector Sequences 33%
• Antibody Protein 33%
• Sequencing Effort 33%
• Tryptic Digestion 33%
• Fragment Ions 33%
• Energy-resolved Collision-induced Dissociation (ER-CID) 33%
• De-novo Sequencing 33%
• Normalized Collision Energy 33%
• Engineered Cells 33%
• Fc-fusion Protein 33%
• State of Charge 33%
• C-terminal Extension 33%

Immunology and Microbiology

• Ovary Cell 100%
• Chinese Hamster 100%
• Carboxy Terminal Sequence 100%
• Liquid Chromatography Mass Spectrometry 40%
• Codon 40%
• Amino Acid Sequence 20%
• Promoter Region 20%
• Protein Processing 20%
• Genetic Transfection 20%
• Engineered Cell 20%
• Immunology 20%

Biochemistry, Genetics and Molecular Biology

• C-Terminus 100%
• Chinese Hamster 100%
• Liquid Chromatography-Mass Spectrometry 40%
• Codon 40%
• Promoter Region 20%
• Posttranslational Modification 20%
• Peptide Sequence 20%
• Mass Spectrometry 20%
• Transfection 20%
• Derived Protein 20%
• Chimeric Protein 20%