Diffusible iodine-based contrast-enhanced computed tomography (diceCT): An emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues

Paul Gignac, Nathan J. Kley, Julia A. Clarke, Matthew W. Colbert, Ashley C. Morhardt, Donald Cerio, Ian N. Cost, Philip G. Cox, Juan D. Daza, Catherine M. Early, M. Scott Echols, R. Mark Henkelman, A. Nele Herdina, Casey M. Holliday, Zhiheng Li, Kristin Mahlow, Samer Merchant, Johannes Müller, Courtney P. Orsbon, Daniel J. PaluhMonte L. Thies, Henry P. Tsai, Lawrence M. Witmer

Research output: Contribution to journalReview article

104 Citations (Scopus)

Abstract

Morphologists have historically had to rely on destructive procedures to visualize the three-dimensional (3-D) anatomy of animals. More recently, however, non-destructive techniques have come to the forefront. These include X-ray computed tomography (CT), which has been used most commonly to examine the mineralized, hard-tissue anatomy of living and fossil metazoans. One relatively new and potentially transformative aspect of current CT-based research is the use of chemical agents to render visible, and differentiate between, soft-tissue structures in X-ray images. Specifically, iodine has emerged as one of the most widely used of these contrast agents among animal morphologists due to its ease of handling, cost effectiveness, and differential affinities for major types of soft tissues. The rapid adoption of iodine-based contrast agents has resulted in a proliferation of distinct specimen preparations and scanning parameter choices, as well as an increasing variety of imaging hardware and software preferences. Here we provide a critical review of the recent contributions to iodine-based, contrast-enhanced CT research to enable researchers just beginning to employ contrast enhancement to make sense of this complex new landscape of methodologies. We provide a detailed summary of recent case studies, assess factors that govern success at each step of the specimen storage, preparation, and imaging processes, and make recommendations for standardizing both techniques and reporting practices. Finally, we discuss potential cutting-edge applications of diffusible iodine-based contrast-enhanced computed tomography (diceCT) and the issues that must still be overcome to facilitate the broader adoption of diceCT going forward.

Original languageEnglish
Pages (from-to)889-909
Number of pages21
JournalJournal of Anatomy
Volume228
Issue number6
DOIs
StatePublished - 1 Jun 2016

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Three-Dimensional Imaging
metazoan
iodine
computed tomography
Iodine
tomography
Tomography
image analysis
anatomy
Contrast Media
Anatomy
X-radiation
animal anatomy
X Ray Computed Tomography
animal
cost effectiveness
Research
hardware
Cost-Benefit Analysis
Software

Keywords

  • Alcoholic iodine
  • Destaining
  • Lugol's iodine
  • Radiographic contrast agents
  • Three-dimensional imaging
  • X-ray micro-CT scanning

Cite this

Gignac, Paul ; Kley, Nathan J. ; Clarke, Julia A. ; Colbert, Matthew W. ; Morhardt, Ashley C. ; Cerio, Donald ; Cost, Ian N. ; Cox, Philip G. ; Daza, Juan D. ; Early, Catherine M. ; Echols, M. Scott ; Henkelman, R. Mark ; Herdina, A. Nele ; Holliday, Casey M. ; Li, Zhiheng ; Mahlow, Kristin ; Merchant, Samer ; Müller, Johannes ; Orsbon, Courtney P. ; Paluh, Daniel J. ; Thies, Monte L. ; Tsai, Henry P. ; Witmer, Lawrence M. / Diffusible iodine-based contrast-enhanced computed tomography (diceCT) : An emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues. In: Journal of Anatomy. 2016 ; Vol. 228, No. 6. pp. 889-909.
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abstract = "Morphologists have historically had to rely on destructive procedures to visualize the three-dimensional (3-D) anatomy of animals. More recently, however, non-destructive techniques have come to the forefront. These include X-ray computed tomography (CT), which has been used most commonly to examine the mineralized, hard-tissue anatomy of living and fossil metazoans. One relatively new and potentially transformative aspect of current CT-based research is the use of chemical agents to render visible, and differentiate between, soft-tissue structures in X-ray images. Specifically, iodine has emerged as one of the most widely used of these contrast agents among animal morphologists due to its ease of handling, cost effectiveness, and differential affinities for major types of soft tissues. The rapid adoption of iodine-based contrast agents has resulted in a proliferation of distinct specimen preparations and scanning parameter choices, as well as an increasing variety of imaging hardware and software preferences. Here we provide a critical review of the recent contributions to iodine-based, contrast-enhanced CT research to enable researchers just beginning to employ contrast enhancement to make sense of this complex new landscape of methodologies. We provide a detailed summary of recent case studies, assess factors that govern success at each step of the specimen storage, preparation, and imaging processes, and make recommendations for standardizing both techniques and reporting practices. Finally, we discuss potential cutting-edge applications of diffusible iodine-based contrast-enhanced computed tomography (diceCT) and the issues that must still be overcome to facilitate the broader adoption of diceCT going forward.",
keywords = "Alcoholic iodine, Destaining, Lugol's iodine, Radiographic contrast agents, Three-dimensional imaging, X-ray micro-CT scanning",
author = "Paul Gignac and Kley, {Nathan J.} and Clarke, {Julia A.} and Colbert, {Matthew W.} and Morhardt, {Ashley C.} and Donald Cerio and Cost, {Ian N.} and Cox, {Philip G.} and Daza, {Juan D.} and Early, {Catherine M.} and Echols, {M. Scott} and Henkelman, {R. Mark} and Herdina, {A. Nele} and Holliday, {Casey M.} and Zhiheng Li and Kristin Mahlow and Samer Merchant and Johannes M{\"u}ller and Orsbon, {Courtney P.} and Paluh, {Daniel J.} and Thies, {Monte L.} and Tsai, {Henry P.} and Witmer, {Lawrence M.}",
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Gignac, P, Kley, NJ, Clarke, JA, Colbert, MW, Morhardt, AC, Cerio, D, Cost, IN, Cox, PG, Daza, JD, Early, CM, Echols, MS, Henkelman, RM, Herdina, AN, Holliday, CM, Li, Z, Mahlow, K, Merchant, S, Müller, J, Orsbon, CP, Paluh, DJ, Thies, ML, Tsai, HP & Witmer, LM 2016, 'Diffusible iodine-based contrast-enhanced computed tomography (diceCT): An emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues', Journal of Anatomy, vol. 228, no. 6, pp. 889-909. https://doi.org/10.1111/joa.12449

Diffusible iodine-based contrast-enhanced computed tomography (diceCT) : An emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues. / Gignac, Paul; Kley, Nathan J.; Clarke, Julia A.; Colbert, Matthew W.; Morhardt, Ashley C.; Cerio, Donald; Cost, Ian N.; Cox, Philip G.; Daza, Juan D.; Early, Catherine M.; Echols, M. Scott; Henkelman, R. Mark; Herdina, A. Nele; Holliday, Casey M.; Li, Zhiheng; Mahlow, Kristin; Merchant, Samer; Müller, Johannes; Orsbon, Courtney P.; Paluh, Daniel J.; Thies, Monte L.; Tsai, Henry P.; Witmer, Lawrence M.

In: Journal of Anatomy, Vol. 228, No. 6, 01.06.2016, p. 889-909.

Research output: Contribution to journalReview article

TY - JOUR

T1 - Diffusible iodine-based contrast-enhanced computed tomography (diceCT)

T2 - An emerging tool for rapid, high-resolution, 3-D imaging of metazoan soft tissues

AU - Gignac, Paul

AU - Kley, Nathan J.

AU - Clarke, Julia A.

AU - Colbert, Matthew W.

AU - Morhardt, Ashley C.

AU - Cerio, Donald

AU - Cost, Ian N.

AU - Cox, Philip G.

AU - Daza, Juan D.

AU - Early, Catherine M.

AU - Echols, M. Scott

AU - Henkelman, R. Mark

AU - Herdina, A. Nele

AU - Holliday, Casey M.

AU - Li, Zhiheng

AU - Mahlow, Kristin

AU - Merchant, Samer

AU - Müller, Johannes

AU - Orsbon, Courtney P.

AU - Paluh, Daniel J.

AU - Thies, Monte L.

AU - Tsai, Henry P.

AU - Witmer, Lawrence M.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Morphologists have historically had to rely on destructive procedures to visualize the three-dimensional (3-D) anatomy of animals. More recently, however, non-destructive techniques have come to the forefront. These include X-ray computed tomography (CT), which has been used most commonly to examine the mineralized, hard-tissue anatomy of living and fossil metazoans. One relatively new and potentially transformative aspect of current CT-based research is the use of chemical agents to render visible, and differentiate between, soft-tissue structures in X-ray images. Specifically, iodine has emerged as one of the most widely used of these contrast agents among animal morphologists due to its ease of handling, cost effectiveness, and differential affinities for major types of soft tissues. The rapid adoption of iodine-based contrast agents has resulted in a proliferation of distinct specimen preparations and scanning parameter choices, as well as an increasing variety of imaging hardware and software preferences. Here we provide a critical review of the recent contributions to iodine-based, contrast-enhanced CT research to enable researchers just beginning to employ contrast enhancement to make sense of this complex new landscape of methodologies. We provide a detailed summary of recent case studies, assess factors that govern success at each step of the specimen storage, preparation, and imaging processes, and make recommendations for standardizing both techniques and reporting practices. Finally, we discuss potential cutting-edge applications of diffusible iodine-based contrast-enhanced computed tomography (diceCT) and the issues that must still be overcome to facilitate the broader adoption of diceCT going forward.

AB - Morphologists have historically had to rely on destructive procedures to visualize the three-dimensional (3-D) anatomy of animals. More recently, however, non-destructive techniques have come to the forefront. These include X-ray computed tomography (CT), which has been used most commonly to examine the mineralized, hard-tissue anatomy of living and fossil metazoans. One relatively new and potentially transformative aspect of current CT-based research is the use of chemical agents to render visible, and differentiate between, soft-tissue structures in X-ray images. Specifically, iodine has emerged as one of the most widely used of these contrast agents among animal morphologists due to its ease of handling, cost effectiveness, and differential affinities for major types of soft tissues. The rapid adoption of iodine-based contrast agents has resulted in a proliferation of distinct specimen preparations and scanning parameter choices, as well as an increasing variety of imaging hardware and software preferences. Here we provide a critical review of the recent contributions to iodine-based, contrast-enhanced CT research to enable researchers just beginning to employ contrast enhancement to make sense of this complex new landscape of methodologies. We provide a detailed summary of recent case studies, assess factors that govern success at each step of the specimen storage, preparation, and imaging processes, and make recommendations for standardizing both techniques and reporting practices. Finally, we discuss potential cutting-edge applications of diffusible iodine-based contrast-enhanced computed tomography (diceCT) and the issues that must still be overcome to facilitate the broader adoption of diceCT going forward.

KW - Alcoholic iodine

KW - Destaining

KW - Lugol's iodine

KW - Radiographic contrast agents

KW - Three-dimensional imaging

KW - X-ray micro-CT scanning

UR - http://www.scopus.com/inward/record.url?scp=84960460037&partnerID=8YFLogxK

U2 - 10.1111/joa.12449

DO - 10.1111/joa.12449

M3 - Review article

C2 - 26970556

AN - SCOPUS:84960460037

VL - 228

SP - 889

EP - 909

JO - Journal of Anatomy

JF - Journal of Anatomy

SN - 0021-8782

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