Differentiation of lactococci by rRNA gene restriction analysis

Gerwald Köhler, Wolfgang Ludwig, Karl Heinz Schleifer

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Strains of the subspecies of Lactococcus lactis could be differentiated by rRNA gene restriction fragment length polymorphisms (RFLP). 16S rRNA-specific oligonucleotide as well as polynucleotide DNA probes were used for the detection of restriction fragments. In addition, a site-specific probe was designed for the intergenic spacer region of 23S and 5S rRNA genes. For all lactococcal strains the putative presence of six rRNA operons was confirmed. A non-radioactive hybridization assay was used based on hybrid detection by chemiluminescence. Specific patterns were found for any of the strains investigated. Subspecies-specific restriction fragments could be identified in addition to the strain-specific patterns.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalFEMS Microbiology Letters
Volume84
Issue number3
DOIs
StatePublished - 1 Dec 1991

Keywords

  • Lactococcus lactis
  • Restriction fragment length polymorphism
  • Ribosomal RNA
  • Strain differentiation

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