Differentiation of lactococci by rRNA gene restriction analysis

Gerwald Köhler; Wolfgang Ludwig; Karl Heinz Schleifer

doi:10.1111/j.1574-6968.1991.tb04615.x

Differentiation of lactococci by rRNA gene restriction analysis

Gerwald Köhler, Wolfgang Ludwig, Karl Heinz Schleifer

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Abstract

Strains of the subspecies of Lactococcus lactis could be differentiated by rRNA gene restriction fragment length polymorphisms (RFLP). 16S rRNA-specific oligonucleotide as well as polynucleotide DNA probes were used for the detection of restriction fragments. In addition, a site-specific probe was designed for the intergenic spacer region of 23S and 5S rRNA genes. For all lactococcal strains the putative presence of six rRNA operons was confirmed. A non-radioactive hybridization assay was used based on hybrid detection by chemiluminescence. Specific patterns were found for any of the strains investigated. Subspecies-specific restriction fragments could be identified in addition to the strain-specific patterns.

Original language	English
Pages (from-to)	307-312
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	84
Issue number	3
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04615.x
State	Published - 1 Dec 1991

Keywords

Lactococcus lactis
Restriction fragment length polymorphism
Ribosomal RNA
Strain differentiation

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10.1111/j.1574-6968.1991.tb04615.x

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AU - Köhler, Gerwald

AU - Ludwig, Wolfgang

AU - Heinz Schleifer, Karl

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N2 - Strains of the subspecies of Lactococcus lactis could be differentiated by rRNA gene restriction fragment length polymorphisms (RFLP). 16S rRNA-specific oligonucleotide as well as polynucleotide DNA probes were used for the detection of restriction fragments. In addition, a site-specific probe was designed for the intergenic spacer region of 23S and 5S rRNA genes. For all lactococcal strains the putative presence of six rRNA operons was confirmed. A non-radioactive hybridization assay was used based on hybrid detection by chemiluminescence. Specific patterns were found for any of the strains investigated. Subspecies-specific restriction fragments could be identified in addition to the strain-specific patterns.

AB - Strains of the subspecies of Lactococcus lactis could be differentiated by rRNA gene restriction fragment length polymorphisms (RFLP). 16S rRNA-specific oligonucleotide as well as polynucleotide DNA probes were used for the detection of restriction fragments. In addition, a site-specific probe was designed for the intergenic spacer region of 23S and 5S rRNA genes. For all lactococcal strains the putative presence of six rRNA operons was confirmed. A non-radioactive hybridization assay was used based on hybrid detection by chemiluminescence. Specific patterns were found for any of the strains investigated. Subspecies-specific restriction fragments could be identified in addition to the strain-specific patterns.

KW - Lactococcus lactis

KW - Restriction fragment length polymorphism

KW - Ribosomal RNA

KW - Strain differentiation

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Differentiation of lactococci by rRNA gene restriction analysis

Abstract

Keywords

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