Differentiation of lactococci by rRNA gene restriction analysis

Gerwald Koehler, Wolfgang Ludwig, Karl Heinz Schleifer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Strains of the subspecies of Lactococcus lactis could be differentiated by rRNA gene restriction fragment length polymorphisms (RFLP). 16S rRNA-specific oligonucleotide as well as polynucleotide DNA probes were used for the detection of restriction fragments. In addition, a site-specific probe was designed for the intergenic spacer region of 23S and 5S rRNA genes. For all lactococcal strains the putative presence of six rRNA operons was confirmed. A non-radioactive hybridization assay was used based on hybrid detection by chemiluminescence. Specific patterns were found for any of the strains investigated. Subspecies-specific restriction fragments could be identified in addition to the strain-specific patterns.

Original languageEnglish
Pages (from-to)307-312
Number of pages6
JournalFEMS Microbiology Letters
Volume84
Issue number3
StatePublished - 1 Dec 1991

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Lactococcus
rRNA Genes
rRNA Operon
Polynucleotides
Lactococcus lactis
Intergenic DNA
DNA Probes
Luminescence
Oligonucleotides
Restriction Fragment Length Polymorphisms

Keywords

  • Lactococcus lactis
  • Restriction fragment length polymorphism
  • Ribosomal RNA
  • Strain differentiation

Cite this

Koehler, Gerwald ; Ludwig, Wolfgang ; Heinz Schleifer, Karl. / Differentiation of lactococci by rRNA gene restriction analysis. In: FEMS Microbiology Letters. 1991 ; Vol. 84, No. 3. pp. 307-312.
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Koehler, G, Ludwig, W & Heinz Schleifer, K 1991, 'Differentiation of lactococci by rRNA gene restriction analysis', FEMS Microbiology Letters, vol. 84, no. 3, pp. 307-312.

Differentiation of lactococci by rRNA gene restriction analysis. / Koehler, Gerwald; Ludwig, Wolfgang; Heinz Schleifer, Karl.

In: FEMS Microbiology Letters, Vol. 84, No. 3, 01.12.1991, p. 307-312.

Research output: Contribution to journalArticle

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