Differential expression of alternative splice variants of β-arrestin-1 and -2 in rat central nervous system and peripheral tissues

Naoka Komori, Sandra D. Cain, Jean Marc Roch, Kenneth Miller, Hiroyuki Matsumoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Members of arrestin/β-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors, including rhodopsin and β2-adrenergic receptor. Unlike in human and cow, splice variants of this protein family in rat have not been studied extensively, and there has been no report on their existence at protein level. Hence, a previous report by others on the localization of both β-arrestin-1 and -2 in a wide range of innervated rat tissues could imply their broad receptor specificity. In this report we show the presence of two alternatively spliced forms of β-arrestin-1 in several rat tissues using both reverse transcription-polymerase chain reaction and Western immunoblot. Splicing of β-arrestin-1 pre-mRNA appears to be subject to differential regulation between the rat CNS and peripheral tissues. In contrast, we detected no splice variants of β-arrestin-2 in rat. A comparison of the genomic DNA sequences of bovine and rat β-arrestin-2, where the splicing of bovine β-arrestin-2 mRNA has been reported, revealed a high degree of homology in their organization of exons and introns as well as certain differences that might be responsible for the different processing of β-arrestin-2 mRNA in the two species. Our two-dimensional isoelectric focusing gels using rat spinal cord and heart tissues demonstrate isoelectric heterogeneity of rat β-arrestin-1, suggesting that β-arrestin-1 is subject to post-translational modification unlike β-arrestin-2.

Original languageEnglish
Pages (from-to)2607-2616
Number of pages10
JournalEuropean Journal of Neuroscience
Volume10
Issue number8
DOIs
StatePublished - 17 Aug 1998

Fingerprint

Arrestin
Central Nervous System
Messenger RNA
Rhodopsin
beta-Arrestin 1
RNA Precursors
Isoelectric Focusing
Post Translational Protein Processing
G-Protein-Coupled Receptors
Introns
Adrenergic Receptors
Reverse Transcription
Exons
Spinal Cord
Protein Isoforms
Proteins
Western Blotting
Gels
Polymerase Chain Reaction

Keywords

  • G-protein-coupled receptor (GPCR)
  • Genomic DNA sequence
  • Isoelectric heterogeneity
  • RT-PCR
  • cDNA sequence

Cite this

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title = "Differential expression of alternative splice variants of β-arrestin-1 and -2 in rat central nervous system and peripheral tissues",
abstract = "Members of arrestin/β-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors, including rhodopsin and β2-adrenergic receptor. Unlike in human and cow, splice variants of this protein family in rat have not been studied extensively, and there has been no report on their existence at protein level. Hence, a previous report by others on the localization of both β-arrestin-1 and -2 in a wide range of innervated rat tissues could imply their broad receptor specificity. In this report we show the presence of two alternatively spliced forms of β-arrestin-1 in several rat tissues using both reverse transcription-polymerase chain reaction and Western immunoblot. Splicing of β-arrestin-1 pre-mRNA appears to be subject to differential regulation between the rat CNS and peripheral tissues. In contrast, we detected no splice variants of β-arrestin-2 in rat. A comparison of the genomic DNA sequences of bovine and rat β-arrestin-2, where the splicing of bovine β-arrestin-2 mRNA has been reported, revealed a high degree of homology in their organization of exons and introns as well as certain differences that might be responsible for the different processing of β-arrestin-2 mRNA in the two species. Our two-dimensional isoelectric focusing gels using rat spinal cord and heart tissues demonstrate isoelectric heterogeneity of rat β-arrestin-1, suggesting that β-arrestin-1 is subject to post-translational modification unlike β-arrestin-2.",
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Differential expression of alternative splice variants of β-arrestin-1 and -2 in rat central nervous system and peripheral tissues. / Komori, Naoka; Cain, Sandra D.; Roch, Jean Marc; Miller, Kenneth; Matsumoto, Hiroyuki.

In: European Journal of Neuroscience, Vol. 10, No. 8, 17.08.1998, p. 2607-2616.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Differential expression of alternative splice variants of β-arrestin-1 and -2 in rat central nervous system and peripheral tissues

AU - Komori, Naoka

AU - Cain, Sandra D.

AU - Roch, Jean Marc

AU - Miller, Kenneth

AU - Matsumoto, Hiroyuki

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