Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae. Mutation in the dra region prevented tubulointerstitial nephritis

Pawel Goluszko, Steve L. Moseley, Luan D. Truong, Anil Kaul, John R. Williford, Rangaraj Selvarangan, Stella Nowicki, Bogdan Nowicki

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Escherichia coli that express Dr fimbriae and related adhesins recognize the common receptor decay accelerating factor. E. coli strains that express adhesins of the Dr family were postulated to be associated with cystitis (30- 50%), pregnancy-associated pyelonephritis (30%), and chronic diarrhea (50%). In this study, we investigated the hypothesis that E. coli renal interstitial binding mediated by the Dr adhesin may be important for the development of chronic pyelonephritis. An insertional dra mutant, E. coli DR14, of the clinical E. coli isolate IH11128 bearing Dr fimbriae, was constructed and used to characterize persistence of infection and interstitial tropism in an experimental model of ascending pyelonephritis. Quantitative cultures of kidney homogenates indicated that Dr hemagglutinin positive (Dr+) E. coli IH 11128 established a 1-yr colonization of renal tissue. In the Dr hemagglutinin negative (Dr-) group, 50% of animals cleared infection within 20 wk and 100% between 32 to 52 wk. Dr+ E. coli colonized the renal interstitium. Significant histological changes corresponding to tubulointerstitial nephritis including interstitial inflammation, fibrosis, and tubular atrophy were found in the kidney tissue of the Dr+ but not the Dr- group. A substantial amount of fimbrial antigen was detected in the parenchymal regions affected by interstitial inflammation and fibrosis. The obtained results are consistent with the hypothesis that mutation within the dra region, affecting E. coli binding to tubular basement membranes, prevented renal interstitial tropism and the development of the changes characteristically seen in tubulointerstitial nephritis.

Original languageEnglish
Pages (from-to)1662-1672
Number of pages11
JournalJournal of Clinical Investigation
Volume99
Issue number7
DOIs
StatePublished - 1 Apr 1997

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Interstitial Nephritis
Pyelonephritis
Theoretical Models
Escherichia coli
Mutation
Kidney
Tropism
Fibrosis
CD55 Antigens
Inflammation
Cystitis
Hemagglutinins
Infection
Basement Membrane
Atrophy
Diarrhea
Antigens
Pregnancy

Keywords

  • Dr fimbriae
  • Escherichia coli
  • attachment
  • collagen type IV
  • decay accelerating factor

Cite this

Goluszko, Pawel ; Moseley, Steve L. ; Truong, Luan D. ; Kaul, Anil ; Williford, John R. ; Selvarangan, Rangaraj ; Nowicki, Stella ; Nowicki, Bogdan. / Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae. Mutation in the dra region prevented tubulointerstitial nephritis. In: Journal of Clinical Investigation. 1997 ; Vol. 99, No. 7. pp. 1662-1672.
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abstract = "Escherichia coli that express Dr fimbriae and related adhesins recognize the common receptor decay accelerating factor. E. coli strains that express adhesins of the Dr family were postulated to be associated with cystitis (30- 50{\%}), pregnancy-associated pyelonephritis (30{\%}), and chronic diarrhea (50{\%}). In this study, we investigated the hypothesis that E. coli renal interstitial binding mediated by the Dr adhesin may be important for the development of chronic pyelonephritis. An insertional dra mutant, E. coli DR14, of the clinical E. coli isolate IH11128 bearing Dr fimbriae, was constructed and used to characterize persistence of infection and interstitial tropism in an experimental model of ascending pyelonephritis. Quantitative cultures of kidney homogenates indicated that Dr hemagglutinin positive (Dr+) E. coli IH 11128 established a 1-yr colonization of renal tissue. In the Dr hemagglutinin negative (Dr-) group, 50{\%} of animals cleared infection within 20 wk and 100{\%} between 32 to 52 wk. Dr+ E. coli colonized the renal interstitium. Significant histological changes corresponding to tubulointerstitial nephritis including interstitial inflammation, fibrosis, and tubular atrophy were found in the kidney tissue of the Dr+ but not the Dr- group. A substantial amount of fimbrial antigen was detected in the parenchymal regions affected by interstitial inflammation and fibrosis. The obtained results are consistent with the hypothesis that mutation within the dra region, affecting E. coli binding to tubular basement membranes, prevented renal interstitial tropism and the development of the changes characteristically seen in tubulointerstitial nephritis.",
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Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae. Mutation in the dra region prevented tubulointerstitial nephritis. / Goluszko, Pawel; Moseley, Steve L.; Truong, Luan D.; Kaul, Anil; Williford, John R.; Selvarangan, Rangaraj; Nowicki, Stella; Nowicki, Bogdan.

In: Journal of Clinical Investigation, Vol. 99, No. 7, 01.04.1997, p. 1662-1672.

Research output: Contribution to journalArticle

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T1 - Development of experimental model of chronic pyelonephritis with Escherichia coli O75:K5:H-bearing Dr fimbriae. Mutation in the dra region prevented tubulointerstitial nephritis

AU - Goluszko, Pawel

AU - Moseley, Steve L.

AU - Truong, Luan D.

AU - Kaul, Anil

AU - Williford, John R.

AU - Selvarangan, Rangaraj

AU - Nowicki, Stella

AU - Nowicki, Bogdan

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AB - Escherichia coli that express Dr fimbriae and related adhesins recognize the common receptor decay accelerating factor. E. coli strains that express adhesins of the Dr family were postulated to be associated with cystitis (30- 50%), pregnancy-associated pyelonephritis (30%), and chronic diarrhea (50%). In this study, we investigated the hypothesis that E. coli renal interstitial binding mediated by the Dr adhesin may be important for the development of chronic pyelonephritis. An insertional dra mutant, E. coli DR14, of the clinical E. coli isolate IH11128 bearing Dr fimbriae, was constructed and used to characterize persistence of infection and interstitial tropism in an experimental model of ascending pyelonephritis. Quantitative cultures of kidney homogenates indicated that Dr hemagglutinin positive (Dr+) E. coli IH 11128 established a 1-yr colonization of renal tissue. In the Dr hemagglutinin negative (Dr-) group, 50% of animals cleared infection within 20 wk and 100% between 32 to 52 wk. Dr+ E. coli colonized the renal interstitium. Significant histological changes corresponding to tubulointerstitial nephritis including interstitial inflammation, fibrosis, and tubular atrophy were found in the kidney tissue of the Dr+ but not the Dr- group. A substantial amount of fimbrial antigen was detected in the parenchymal regions affected by interstitial inflammation and fibrosis. The obtained results are consistent with the hypothesis that mutation within the dra region, affecting E. coli binding to tubular basement membranes, prevented renal interstitial tropism and the development of the changes characteristically seen in tubulointerstitial nephritis.

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