Development of a competitive ELISA for detection of primates infected with monkey B virus (Herpesvirus simiae)

Earl Linwood Blewett, Jeremiah T. Saliki, R. Eberle

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Two competitive ELISAs (C-ELISAs) are described that allow detection of antibodies against monkey B virus (BV, Cercopithecine herpesvirus 1). The assays utilize monoclonal antibodies (MABs) directed against the BV glycoprotein B (gB). Two of these MABs specifically recognize BV gB while a third MAB also reacts with the gB homologues of other primate α-herpesviruses (herpes simplexvirus-1, HSV-1; HSV-2; simian agent-8, SA8; and Herpesvirus papio-2, HVP2). A C-ELISA using the single cross-reactive MAB 3E8 allowed detection of host antibodies against HSV-1, HSV-2, SA8, HVP2 or BV, thus proving to be a sensitive assay for the detection of infection by any of these primate α-herpesviruses. The C-ELISA using BV-specific MABs was less sensitive but did allow some discrimination between infection by BV versus other α-herpesviruses. It was also shown that a C-ELISA using HVP2 as antigen and the cross-reactive MAB 3E8 was as sensitive for detection of BV antibody in macaque sera as an assay employing BV antigen. This test format allows detection of BV-infected primates without the biohazards associated with preparation and use of BV antigen. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)59-67
Number of pages9
JournalJournal of Virological Methods
Volume77
Issue number1
DOIs
StatePublished - 1 Jan 1999

Keywords

  • BV
  • ELISA
  • Herpesvirus simiae
  • Macaque
  • Monkey B virus

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