TY - JOUR
T1 - Development of a competitive ELISA for detection of primates infected with monkey B virus (Herpesvirus simiae)
AU - Linwood Blewett, Earl
AU - Saliki, Jeremiah T.
AU - Eberle, R.
PY - 1999/1/1
Y1 - 1999/1/1
N2 - Two competitive ELISAs (C-ELISAs) are described that allow detection of antibodies against monkey B virus (BV, Cercopithecine herpesvirus 1). The assays utilize monoclonal antibodies (MABs) directed against the BV glycoprotein B (gB). Two of these MABs specifically recognize BV gB while a third MAB also reacts with the gB homologues of other primate α-herpesviruses (herpes simplexvirus-1, HSV-1; HSV-2; simian agent-8, SA8; and Herpesvirus papio-2, HVP2). A C-ELISA using the single cross-reactive MAB 3E8 allowed detection of host antibodies against HSV-1, HSV-2, SA8, HVP2 or BV, thus proving to be a sensitive assay for the detection of infection by any of these primate α-herpesviruses. The C-ELISA using BV-specific MABs was less sensitive but did allow some discrimination between infection by BV versus other α-herpesviruses. It was also shown that a C-ELISA using HVP2 as antigen and the cross-reactive MAB 3E8 was as sensitive for detection of BV antibody in macaque sera as an assay employing BV antigen. This test format allows detection of BV-infected primates without the biohazards associated with preparation and use of BV antigen. Copyright (C) 1999 Elsevier Science B.V.
AB - Two competitive ELISAs (C-ELISAs) are described that allow detection of antibodies against monkey B virus (BV, Cercopithecine herpesvirus 1). The assays utilize monoclonal antibodies (MABs) directed against the BV glycoprotein B (gB). Two of these MABs specifically recognize BV gB while a third MAB also reacts with the gB homologues of other primate α-herpesviruses (herpes simplexvirus-1, HSV-1; HSV-2; simian agent-8, SA8; and Herpesvirus papio-2, HVP2). A C-ELISA using the single cross-reactive MAB 3E8 allowed detection of host antibodies against HSV-1, HSV-2, SA8, HVP2 or BV, thus proving to be a sensitive assay for the detection of infection by any of these primate α-herpesviruses. The C-ELISA using BV-specific MABs was less sensitive but did allow some discrimination between infection by BV versus other α-herpesviruses. It was also shown that a C-ELISA using HVP2 as antigen and the cross-reactive MAB 3E8 was as sensitive for detection of BV antibody in macaque sera as an assay employing BV antigen. This test format allows detection of BV-infected primates without the biohazards associated with preparation and use of BV antigen. Copyright (C) 1999 Elsevier Science B.V.
KW - BV
KW - ELISA
KW - Herpesvirus simiae
KW - Macaque
KW - Monkey B virus
UR - http://www.scopus.com/inward/record.url?scp=0032905081&partnerID=8YFLogxK
U2 - 10.1016/S0166-0934(98)00134-7
DO - 10.1016/S0166-0934(98)00134-7
M3 - Article
C2 - 10029325
AN - SCOPUS:0032905081
SN - 0166-0934
VL - 77
SP - 59
EP - 67
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -