Determination of an Effective Housekeeping Gene for the Quantification of mRNA for Forensic Applications

Lilliana I. Moreno, Courtney M. Tate, Erika L. Knott, Jade E. McDaniel, Stephanie Rogers, Barbara W. Koons, Mark F. Kavlick, Rhonda L. Craig, James M. Robertson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The potential application of mRNA for the identification of biological fluids using molecular techniques has been a recent development in forensic serology. Constitutively expressed housekeeping genes can assess the amount of mRNA recovered from a sample, establish its suitability for downstream applications, and provide a reference point to corroborate the identity of the fluid. qPCR was utilized to compare the expression levels of housekeeping genes from forensic-like body fluid stains to establish the most appropriate assessment of human mRNA quantity prior to profiling. Although variability was observed between fluids and individuals, results indicated that beta-2 microglobulin exhibited the highest expression for all body fluids examined and across donors. A one-way analysis of variance was performed for housekeeping gene variability between donors (at the α, 0.05, significance level), and the results indicated significant differences for semen, vaginal secretions, and menstrual blood.
Original languageAmerican English
Pages (from-to)1051-58
JournalJournal of Forensic Sciences
Volume57
Issue number4
DOIs
StatePublished - 6 Feb 2012

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