Detection of venom factors releasing histamine from rat peritoneal mast cells

J. A. Price, G. G. Sanny

Research output: Contribution to journalArticle

Abstract

Venoms are known to contain many types of toxins. Some show direct cytotoxicity but not always to the same extent on each type of target cell tested. These experiments asked if venoms might be a source of factors which release histamine from mast cells. These factors would be proinflammatory and could thus significantly contribute to the pathology of snake bite envenomation. These might be either direct lytic factors described by their action on other cells but now found to be active on mast cells, or be previously undescribed, since the direct effects of venoms on mast cells have not been extensively explored. After establishing that a wide variety of venoms were effective at releasing histamine in this system, the toxin fractions (peptides below 10KD prepared using molecular filtration membranes) were examined both for proportion of total venom (based on A214 and A280 ) and for histamine releasing activity. Of the forty venoms tested, the cobra venoms were most active, showing kinetics of activity consistent with peptide toxins. One cobra venom selected for size exclusion analysis contained a unique low molecular weight peak of activity in addition to what is presumably the known cytotoxins. Other venoms in several phylogenetic groups also showed significant activity. These studies: indicate that cobra venom peptides of the size of the cobra cytotoxins are active upon mast cells; that also active are other venom toxins which may be factors known for activity on other cells or known by another activity altogether than cytotoxicity; and are consistent with the interpretation that there may also be other significant undescribed histamine releasing factors present among venoms.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1 Dec 1996

Fingerprint

Venoms
venoms
mast cells
histamine
Mast Cells
Rats
rats
Cobra Venoms
Elapidae
Histamine
Snake Bites
Cytotoxins
toxins
Cytotoxicity
Peptides
fenflumizole
cytotoxins
Cobra Cardiotoxin Proteins
peptides
translationally-controlled 1 tumor protein

Cite this

@article{a067ec3021894bc6b0cd829674416261,
title = "Detection of venom factors releasing histamine from rat peritoneal mast cells",
abstract = "Venoms are known to contain many types of toxins. Some show direct cytotoxicity but not always to the same extent on each type of target cell tested. These experiments asked if venoms might be a source of factors which release histamine from mast cells. These factors would be proinflammatory and could thus significantly contribute to the pathology of snake bite envenomation. These might be either direct lytic factors described by their action on other cells but now found to be active on mast cells, or be previously undescribed, since the direct effects of venoms on mast cells have not been extensively explored. After establishing that a wide variety of venoms were effective at releasing histamine in this system, the toxin fractions (peptides below 10KD prepared using molecular filtration membranes) were examined both for proportion of total venom (based on A214 and A280 ) and for histamine releasing activity. Of the forty venoms tested, the cobra venoms were most active, showing kinetics of activity consistent with peptide toxins. One cobra venom selected for size exclusion analysis contained a unique low molecular weight peak of activity in addition to what is presumably the known cytotoxins. Other venoms in several phylogenetic groups also showed significant activity. These studies: indicate that cobra venom peptides of the size of the cobra cytotoxins are active upon mast cells; that also active are other venom toxins which may be factors known for activity on other cells or known by another activity altogether than cytotoxicity; and are consistent with the interpretation that there may also be other significant undescribed histamine releasing factors present among venoms.",
author = "Price, {J. A.} and Sanny, {G. G.}",
year = "1996",
month = "12",
day = "1",
language = "English",
volume = "10",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

Detection of venom factors releasing histamine from rat peritoneal mast cells. / Price, J. A.; Sanny, G. G.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Detection of venom factors releasing histamine from rat peritoneal mast cells

AU - Price, J. A.

AU - Sanny, G. G.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Venoms are known to contain many types of toxins. Some show direct cytotoxicity but not always to the same extent on each type of target cell tested. These experiments asked if venoms might be a source of factors which release histamine from mast cells. These factors would be proinflammatory and could thus significantly contribute to the pathology of snake bite envenomation. These might be either direct lytic factors described by their action on other cells but now found to be active on mast cells, or be previously undescribed, since the direct effects of venoms on mast cells have not been extensively explored. After establishing that a wide variety of venoms were effective at releasing histamine in this system, the toxin fractions (peptides below 10KD prepared using molecular filtration membranes) were examined both for proportion of total venom (based on A214 and A280 ) and for histamine releasing activity. Of the forty venoms tested, the cobra venoms were most active, showing kinetics of activity consistent with peptide toxins. One cobra venom selected for size exclusion analysis contained a unique low molecular weight peak of activity in addition to what is presumably the known cytotoxins. Other venoms in several phylogenetic groups also showed significant activity. These studies: indicate that cobra venom peptides of the size of the cobra cytotoxins are active upon mast cells; that also active are other venom toxins which may be factors known for activity on other cells or known by another activity altogether than cytotoxicity; and are consistent with the interpretation that there may also be other significant undescribed histamine releasing factors present among venoms.

AB - Venoms are known to contain many types of toxins. Some show direct cytotoxicity but not always to the same extent on each type of target cell tested. These experiments asked if venoms might be a source of factors which release histamine from mast cells. These factors would be proinflammatory and could thus significantly contribute to the pathology of snake bite envenomation. These might be either direct lytic factors described by their action on other cells but now found to be active on mast cells, or be previously undescribed, since the direct effects of venoms on mast cells have not been extensively explored. After establishing that a wide variety of venoms were effective at releasing histamine in this system, the toxin fractions (peptides below 10KD prepared using molecular filtration membranes) were examined both for proportion of total venom (based on A214 and A280 ) and for histamine releasing activity. Of the forty venoms tested, the cobra venoms were most active, showing kinetics of activity consistent with peptide toxins. One cobra venom selected for size exclusion analysis contained a unique low molecular weight peak of activity in addition to what is presumably the known cytotoxins. Other venoms in several phylogenetic groups also showed significant activity. These studies: indicate that cobra venom peptides of the size of the cobra cytotoxins are active upon mast cells; that also active are other venom toxins which may be factors known for activity on other cells or known by another activity altogether than cytotoxicity; and are consistent with the interpretation that there may also be other significant undescribed histamine releasing factors present among venoms.

UR - http://www.scopus.com/inward/record.url?scp=33749111880&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749111880

VL - 10

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -