Detection of baboon cytomegalovirus (BaCMV) by PCR using primers directed against the glycoprotein B gene

Tami G. Ross, Robert P. Rogers, Nathan Elfrink, Natasha Bray, Earl Blewett

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have cloned and sequenced the glycoprotein B genes from five strains of BaCMV, isolated from three subspecies of cynocephalus baboons (olive, yellow and chacma). Primers were designed using conserved DNA regions of the gB gene to allow DNA amplification from all strains of BaCMV. These regions differ sufficiently from human CMV that HCMV strains are not amplified, thus allowing differentiation of BaCMV from HCMV. These diagnostic primers were used to test crude nucleic acid extracts from 27 strains of BaCMV and detected 26 of them. Overall, the sensitivity and specificity of this assay are 96.7 and 100%, respectively. BaCMV strains isolated from yellow and olive baboons were very similar and could be discriminated from strains isolated from chacma baboons using a second set of PCR primers. Phylogenetic analysis of the gB genes supported the inferred close relationship of strains isolated from olive and yellow baboons.

Original languageEnglish
Pages (from-to)119-124
Number of pages6
JournalJournal of Virological Methods
Volume125
Issue number2
DOIs
StatePublished - 1 Jan 2005

Fingerprint

Papio
Cytomegalovirus
Papio cynocephalus
Papio anubis
Glycoproteins
Papio ursinus
Polymerase Chain Reaction
Genes
DNA
Nucleic Acids
Sensitivity and Specificity

Keywords

  • BaCMV
  • Baboon
  • Cytomegalovirus
  • Herpesvirus

Cite this

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abstract = "We have cloned and sequenced the glycoprotein B genes from five strains of BaCMV, isolated from three subspecies of cynocephalus baboons (olive, yellow and chacma). Primers were designed using conserved DNA regions of the gB gene to allow DNA amplification from all strains of BaCMV. These regions differ sufficiently from human CMV that HCMV strains are not amplified, thus allowing differentiation of BaCMV from HCMV. These diagnostic primers were used to test crude nucleic acid extracts from 27 strains of BaCMV and detected 26 of them. Overall, the sensitivity and specificity of this assay are 96.7 and 100{\%}, respectively. BaCMV strains isolated from yellow and olive baboons were very similar and could be discriminated from strains isolated from chacma baboons using a second set of PCR primers. Phylogenetic analysis of the gB genes supported the inferred close relationship of strains isolated from olive and yellow baboons.",
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Detection of baboon cytomegalovirus (BaCMV) by PCR using primers directed against the glycoprotein B gene. / Ross, Tami G.; Rogers, Robert P.; Elfrink, Nathan; Bray, Natasha; Blewett, Earl.

In: Journal of Virological Methods, Vol. 125, No. 2, 01.01.2005, p. 119-124.

Research output: Contribution to journalArticle

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