Defective virions of simian adenovirus 7 produced by one passage at high multiplicity of infection

Joseph A. Price; Leonard C. Norkin

doi:10.1016/0042-6822(78)90367-7

Defective virions of simian adenovirus 7 produced by one passage at high multiplicity of infection

Joseph A. Price, Leonard C. Norkin

Pathology

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Abstract

An assay is described for the rapid microtitration of simian adenovirus 7 (SA7) particles capable of inducing adenovirus CPE (rounded, swollen cells). Utilizing the assay for cytopathic particles, it was found that only one passage of standard SA7 at high m.o.i. results in virus stocks containing an excess of noninfectious cytopathic particles. These stocks also contain an excess of defective virions which retain the ability to enhance the replication of human adenovirus type 5 during mixed infection of monkey cells. SA7 stocks produced on two SV40-transformed simian cell lines contained larger fractions of these defective virions than stocks produced on normal simian cell lines. Autointerference activity was not observed in SA7 stocks containing an excess of defective cytopathic particles nor were there detectable levels of shortened viral genomes.

Original language	English
Pages (from-to)	194-197
Number of pages	4
Journal	Virology
Volume	91
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(78)90367-7
State	Published - Nov 1978

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title = "Defective virions of simian adenovirus 7 produced by one passage at high multiplicity of infection",

abstract = "An assay is described for the rapid microtitration of simian adenovirus 7 (SA7) particles capable of inducing adenovirus CPE (rounded, swollen cells). Utilizing the assay for cytopathic particles, it was found that only one passage of standard SA7 at high m.o.i. results in virus stocks containing an excess of noninfectious cytopathic particles. These stocks also contain an excess of defective virions which retain the ability to enhance the replication of human adenovirus type 5 during mixed infection of monkey cells. SA7 stocks produced on two SV40-transformed simian cell lines contained larger fractions of these defective virions than stocks produced on normal simian cell lines. Autointerference activity was not observed in SA7 stocks containing an excess of defective cytopathic particles nor were there detectable levels of shortened viral genomes.",

author = "Price, {Joseph A.} and Norkin, {Leonard C.}",

note = "Funding Information: This investigation was supported in part by Public Health Service Grants 1 R01 AI14049 from the National Institute of Allergy and Infectious Diseases and Biomedical Research Support Grant RR 07648 and by a grant from the American Cancer Society, Massachusetts Division, Inc.",

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AU - Norkin, Leonard C.

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N2 - An assay is described for the rapid microtitration of simian adenovirus 7 (SA7) particles capable of inducing adenovirus CPE (rounded, swollen cells). Utilizing the assay for cytopathic particles, it was found that only one passage of standard SA7 at high m.o.i. results in virus stocks containing an excess of noninfectious cytopathic particles. These stocks also contain an excess of defective virions which retain the ability to enhance the replication of human adenovirus type 5 during mixed infection of monkey cells. SA7 stocks produced on two SV40-transformed simian cell lines contained larger fractions of these defective virions than stocks produced on normal simian cell lines. Autointerference activity was not observed in SA7 stocks containing an excess of defective cytopathic particles nor were there detectable levels of shortened viral genomes.

AB - An assay is described for the rapid microtitration of simian adenovirus 7 (SA7) particles capable of inducing adenovirus CPE (rounded, swollen cells). Utilizing the assay for cytopathic particles, it was found that only one passage of standard SA7 at high m.o.i. results in virus stocks containing an excess of noninfectious cytopathic particles. These stocks also contain an excess of defective virions which retain the ability to enhance the replication of human adenovirus type 5 during mixed infection of monkey cells. SA7 stocks produced on two SV40-transformed simian cell lines contained larger fractions of these defective virions than stocks produced on normal simian cell lines. Autointerference activity was not observed in SA7 stocks containing an excess of defective cytopathic particles nor were there detectable levels of shortened viral genomes.

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Defective virions of simian adenovirus 7 produced by one passage at high multiplicity of infection

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