Decay-accelerating factor is expressed is the human endometrium and may serve as the attachment ligand for Dr pili of Escherichia coli

Anil Kaul, B. J. Nowicki, M. G. Martens, P. Goluszko, A. Hart, M. Nagamani, D. Kumar, T. Q. Pham, S. Nowicki

Research output: Contribution to journalArticle

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Abstract

PROBLEM: We evaluated the hypothesis that different tissue substructures in uteri may express decay accelerating factor (DAF), a complement regulatory protein that also may serve as ligand for bacterial attachment. METHOD: Purified Dr pili, anti-Dr pili IgG, anti-DAF (SCR-3) IgG, and fluorescein-isothiocyanate-conjugated secondary IgG were used for binding and inhibition experiments. RESULT: We observed staining of endometrial glands, spiral arterioles, and myometrial arteries with Dr adhesin (pili) and anti-DAF (SCR-3) IgG, and found variation in distribution and amount of Dr ligands in different individuals. Anti-DAF (SCR-3) IgG blocked the binding of Dr pili to the endometrium. CONCLUSION: Presence of DAF in endometrium may protect tissues from complement-induced damage. Differences between individuals in DAF density in the endometrium may affect sensitivity to attachment of Dr-bearing E. coli and/or complement activation.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalAmerican Journal of Reproductive Immunology
Volume32
Issue number3
StatePublished - 1 Dec 1994

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CD55 Antigens
Endometrium
Escherichia coli
Ligands
Immunoglobulin G
Complement Activation
Arterioles
Fluorescein
Individuality
Uterus
Complement System Proteins
Arteries
Staining and Labeling

Keywords

  • Colonization
  • DAF
  • Dr fimbriae
  • Dr ligands
  • E. coli
  • Urogenital tract

Cite this

Kaul, Anil ; Nowicki, B. J. ; Martens, M. G. ; Goluszko, P. ; Hart, A. ; Nagamani, M. ; Kumar, D. ; Pham, T. Q. ; Nowicki, S. / Decay-accelerating factor is expressed is the human endometrium and may serve as the attachment ligand for Dr pili of Escherichia coli. In: American Journal of Reproductive Immunology. 1994 ; Vol. 32, No. 3. pp. 194-199.
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abstract = "PROBLEM: We evaluated the hypothesis that different tissue substructures in uteri may express decay accelerating factor (DAF), a complement regulatory protein that also may serve as ligand for bacterial attachment. METHOD: Purified Dr pili, anti-Dr pili IgG, anti-DAF (SCR-3) IgG, and fluorescein-isothiocyanate-conjugated secondary IgG were used for binding and inhibition experiments. RESULT: We observed staining of endometrial glands, spiral arterioles, and myometrial arteries with Dr adhesin (pili) and anti-DAF (SCR-3) IgG, and found variation in distribution and amount of Dr ligands in different individuals. Anti-DAF (SCR-3) IgG blocked the binding of Dr pili to the endometrium. CONCLUSION: Presence of DAF in endometrium may protect tissues from complement-induced damage. Differences between individuals in DAF density in the endometrium may affect sensitivity to attachment of Dr-bearing E. coli and/or complement activation.",
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author = "Anil Kaul and Nowicki, {B. J.} and Martens, {M. G.} and P. Goluszko and A. Hart and M. Nagamani and D. Kumar and Pham, {T. Q.} and S. Nowicki",
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Kaul, A, Nowicki, BJ, Martens, MG, Goluszko, P, Hart, A, Nagamani, M, Kumar, D, Pham, TQ & Nowicki, S 1994, 'Decay-accelerating factor is expressed is the human endometrium and may serve as the attachment ligand for Dr pili of Escherichia coli', American Journal of Reproductive Immunology, vol. 32, no. 3, pp. 194-199.

Decay-accelerating factor is expressed is the human endometrium and may serve as the attachment ligand for Dr pili of Escherichia coli. / Kaul, Anil; Nowicki, B. J.; Martens, M. G.; Goluszko, P.; Hart, A.; Nagamani, M.; Kumar, D.; Pham, T. Q.; Nowicki, S.

In: American Journal of Reproductive Immunology, Vol. 32, No. 3, 01.12.1994, p. 194-199.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Decay-accelerating factor is expressed is the human endometrium and may serve as the attachment ligand for Dr pili of Escherichia coli

AU - Kaul, Anil

AU - Nowicki, B. J.

AU - Martens, M. G.

AU - Goluszko, P.

AU - Hart, A.

AU - Nagamani, M.

AU - Kumar, D.

AU - Pham, T. Q.

AU - Nowicki, S.

PY - 1994/12/1

Y1 - 1994/12/1

N2 - PROBLEM: We evaluated the hypothesis that different tissue substructures in uteri may express decay accelerating factor (DAF), a complement regulatory protein that also may serve as ligand for bacterial attachment. METHOD: Purified Dr pili, anti-Dr pili IgG, anti-DAF (SCR-3) IgG, and fluorescein-isothiocyanate-conjugated secondary IgG were used for binding and inhibition experiments. RESULT: We observed staining of endometrial glands, spiral arterioles, and myometrial arteries with Dr adhesin (pili) and anti-DAF (SCR-3) IgG, and found variation in distribution and amount of Dr ligands in different individuals. Anti-DAF (SCR-3) IgG blocked the binding of Dr pili to the endometrium. CONCLUSION: Presence of DAF in endometrium may protect tissues from complement-induced damage. Differences between individuals in DAF density in the endometrium may affect sensitivity to attachment of Dr-bearing E. coli and/or complement activation.

AB - PROBLEM: We evaluated the hypothesis that different tissue substructures in uteri may express decay accelerating factor (DAF), a complement regulatory protein that also may serve as ligand for bacterial attachment. METHOD: Purified Dr pili, anti-Dr pili IgG, anti-DAF (SCR-3) IgG, and fluorescein-isothiocyanate-conjugated secondary IgG were used for binding and inhibition experiments. RESULT: We observed staining of endometrial glands, spiral arterioles, and myometrial arteries with Dr adhesin (pili) and anti-DAF (SCR-3) IgG, and found variation in distribution and amount of Dr ligands in different individuals. Anti-DAF (SCR-3) IgG blocked the binding of Dr pili to the endometrium. CONCLUSION: Presence of DAF in endometrium may protect tissues from complement-induced damage. Differences between individuals in DAF density in the endometrium may affect sensitivity to attachment of Dr-bearing E. coli and/or complement activation.

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KW - DAF

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