Cross-clade inhibition of human immunodeficiency virus type 1 primary isolates by monoclonal anti-CD4

Michael H. Shearer, Dusti K. Timanus, Patricia A. Benton, D. Rick Lee, Ronald C. Kennedy

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A murine monoclonal antibody (MAb) with human CD4 specificity was tested for the ability to inhibit primary human immunodeficiency virus type 1 (HIV- 1) isolates clades A through E. Human peripheral blood mononuclear cells (PBMC) were used as target cells for infectivity. The HIV-1 primary isolates were examined for the capacity to infect PBMC targets in the presence or absence of the anti-CD4 MAb, designated P1. P1 broadly inhibited clade A, C, D, and E isolates, based on a reduction of HIV-1 p24 antigen concentrations compared with untreated controls. Little to no virus-inhibiting activity was observed with a primary HIV-1 clade B isolate, designated BZ167. Additionally, a second primary clade B isolate was efficiently inhibited from infecting PBMC targets by P1. The data indicate that P1 exhibits group- specific inhibiting activity against non-clade B primary HIV-1 isolates in vitro.

Original languageEnglish
Pages (from-to)1727-1729
Number of pages3
JournalJournal of Infectious Diseases
Volume177
Issue number6
DOIs
StatePublished - 1998

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