TY - JOUR
T1 - Cross-clade inhibition of human immunodeficiency virus type 1 primary isolates by monoclonal anti-CD4
AU - Shearer, Michael H.
AU - Timanus, Dusti K.
AU - Benton, Patricia A.
AU - Lee, D. Rick
AU - Kennedy, Ronald C.
N1 - Funding Information:
Received 28 July 1997; revised 14 November 1997. Grant support: NIH (CA-74101). Reprints or correspondence: Dr. Ronald C. Kennedy, Dept. of Microbiology and Immunology, University of Oklahoma Health Sciences Center, 940 Stanton L. Young Blvd, Oklahoma City, OK 73190 ([email protected]).
PY - 1998
Y1 - 1998
N2 - A murine monoclonal antibody (MAb) with human CD4 specificity was tested for the ability to inhibit primary human immunodeficiency virus type 1 (HIV- 1) isolates clades A through E. Human peripheral blood mononuclear cells (PBMC) were used as target cells for infectivity. The HIV-1 primary isolates were examined for the capacity to infect PBMC targets in the presence or absence of the anti-CD4 MAb, designated P1. P1 broadly inhibited clade A, C, D, and E isolates, based on a reduction of HIV-1 p24 antigen concentrations compared with untreated controls. Little to no virus-inhibiting activity was observed with a primary HIV-1 clade B isolate, designated BZ167. Additionally, a second primary clade B isolate was efficiently inhibited from infecting PBMC targets by P1. The data indicate that P1 exhibits group- specific inhibiting activity against non-clade B primary HIV-1 isolates in vitro.
AB - A murine monoclonal antibody (MAb) with human CD4 specificity was tested for the ability to inhibit primary human immunodeficiency virus type 1 (HIV- 1) isolates clades A through E. Human peripheral blood mononuclear cells (PBMC) were used as target cells for infectivity. The HIV-1 primary isolates were examined for the capacity to infect PBMC targets in the presence or absence of the anti-CD4 MAb, designated P1. P1 broadly inhibited clade A, C, D, and E isolates, based on a reduction of HIV-1 p24 antigen concentrations compared with untreated controls. Little to no virus-inhibiting activity was observed with a primary HIV-1 clade B isolate, designated BZ167. Additionally, a second primary clade B isolate was efficiently inhibited from infecting PBMC targets by P1. The data indicate that P1 exhibits group- specific inhibiting activity against non-clade B primary HIV-1 isolates in vitro.
UR - http://www.scopus.com/inward/record.url?scp=0031779312&partnerID=8YFLogxK
U2 - 10.1086/517432
DO - 10.1086/517432
M3 - Article
C2 - 9607858
AN - SCOPUS:0031779312
SN - 0022-1899
VL - 177
SP - 1727
EP - 1729
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -