Conversion of phospholipids to free fatty acids in response to acquisition of polymyxin resistance in Pseudomonas aeruginosa

Franklin Champlin, H. E. Gilleland, R. S. Conrad

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The readily extractable lipids from a Pseudomonas aeruginosa isolate stepwse adapted to polymyxin resistance were compared with those of the susceptible parent and of a revertant strain which regained susceptibility. Significant qualitative and quantitative lipid alterations accompany the acquisition of resistance. Changes include the appearance of a major unidentified lipid (lipid X) unique to the readily extractable lipids of resistent cells. Comparative studies with parent and revertant strains indicated a significant decrease in the phospholipid content of resistant cells. Thin-layer chromatography of resistant-cell readily extractable lipids demonstrated: (i) the emergence of lipid X (36% of total readily extractable lipids), (ii) a decrease in phosphatidylethanolamine and phosphatidylglycerol, and (iii) an increase in diphosphatidylglycerol. Lipid X was purified by preparative silicic acid column chromatography and thin-layer chromatography and characterized by analytical thin-layer chromatography, column adsorption chromatography, and gas-liquid chromatography. Data from this study indicated that lipid X was a mixture of free fatty acids. The fatty acids present in lipid X were qualitatively and quantitatively the same as the fatty acids esterified to the phospholipids in the readily extractable lipids.

Original languageEnglish
Pages (from-to)5-9
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume24
Issue number1
DOIs
StatePublished - 1 Jan 1983

Fingerprint

Polymyxins
Nonesterified Fatty Acids
Pseudomonas aeruginosa
Phospholipids
Lipids
Thin Layer Chromatography
Chromatography
Fatty Acids
Silicic Acid
Phosphatidylglycerols
Cardiolipins
Gas Chromatography
Adsorption
lipid X

Cite this

@article{6b10464a009247a6bf792afbb6b10794,
title = "Conversion of phospholipids to free fatty acids in response to acquisition of polymyxin resistance in Pseudomonas aeruginosa",
abstract = "The readily extractable lipids from a Pseudomonas aeruginosa isolate stepwse adapted to polymyxin resistance were compared with those of the susceptible parent and of a revertant strain which regained susceptibility. Significant qualitative and quantitative lipid alterations accompany the acquisition of resistance. Changes include the appearance of a major unidentified lipid (lipid X) unique to the readily extractable lipids of resistent cells. Comparative studies with parent and revertant strains indicated a significant decrease in the phospholipid content of resistant cells. Thin-layer chromatography of resistant-cell readily extractable lipids demonstrated: (i) the emergence of lipid X (36{\%} of total readily extractable lipids), (ii) a decrease in phosphatidylethanolamine and phosphatidylglycerol, and (iii) an increase in diphosphatidylglycerol. Lipid X was purified by preparative silicic acid column chromatography and thin-layer chromatography and characterized by analytical thin-layer chromatography, column adsorption chromatography, and gas-liquid chromatography. Data from this study indicated that lipid X was a mixture of free fatty acids. The fatty acids present in lipid X were qualitatively and quantitatively the same as the fatty acids esterified to the phospholipids in the readily extractable lipids.",
author = "Franklin Champlin and Gilleland, {H. E.} and Conrad, {R. S.}",
year = "1983",
month = "1",
day = "1",
doi = "10.1128/AAC.24.1.5",
language = "English",
volume = "24",
pages = "5--9",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "1",

}

Conversion of phospholipids to free fatty acids in response to acquisition of polymyxin resistance in Pseudomonas aeruginosa. / Champlin, Franklin; Gilleland, H. E.; Conrad, R. S.

In: Antimicrobial Agents and Chemotherapy, Vol. 24, No. 1, 01.01.1983, p. 5-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Conversion of phospholipids to free fatty acids in response to acquisition of polymyxin resistance in Pseudomonas aeruginosa

AU - Champlin, Franklin

AU - Gilleland, H. E.

AU - Conrad, R. S.

PY - 1983/1/1

Y1 - 1983/1/1

N2 - The readily extractable lipids from a Pseudomonas aeruginosa isolate stepwse adapted to polymyxin resistance were compared with those of the susceptible parent and of a revertant strain which regained susceptibility. Significant qualitative and quantitative lipid alterations accompany the acquisition of resistance. Changes include the appearance of a major unidentified lipid (lipid X) unique to the readily extractable lipids of resistent cells. Comparative studies with parent and revertant strains indicated a significant decrease in the phospholipid content of resistant cells. Thin-layer chromatography of resistant-cell readily extractable lipids demonstrated: (i) the emergence of lipid X (36% of total readily extractable lipids), (ii) a decrease in phosphatidylethanolamine and phosphatidylglycerol, and (iii) an increase in diphosphatidylglycerol. Lipid X was purified by preparative silicic acid column chromatography and thin-layer chromatography and characterized by analytical thin-layer chromatography, column adsorption chromatography, and gas-liquid chromatography. Data from this study indicated that lipid X was a mixture of free fatty acids. The fatty acids present in lipid X were qualitatively and quantitatively the same as the fatty acids esterified to the phospholipids in the readily extractable lipids.

AB - The readily extractable lipids from a Pseudomonas aeruginosa isolate stepwse adapted to polymyxin resistance were compared with those of the susceptible parent and of a revertant strain which regained susceptibility. Significant qualitative and quantitative lipid alterations accompany the acquisition of resistance. Changes include the appearance of a major unidentified lipid (lipid X) unique to the readily extractable lipids of resistent cells. Comparative studies with parent and revertant strains indicated a significant decrease in the phospholipid content of resistant cells. Thin-layer chromatography of resistant-cell readily extractable lipids demonstrated: (i) the emergence of lipid X (36% of total readily extractable lipids), (ii) a decrease in phosphatidylethanolamine and phosphatidylglycerol, and (iii) an increase in diphosphatidylglycerol. Lipid X was purified by preparative silicic acid column chromatography and thin-layer chromatography and characterized by analytical thin-layer chromatography, column adsorption chromatography, and gas-liquid chromatography. Data from this study indicated that lipid X was a mixture of free fatty acids. The fatty acids present in lipid X were qualitatively and quantitatively the same as the fatty acids esterified to the phospholipids in the readily extractable lipids.

UR - http://www.scopus.com/inward/record.url?scp=0020639056&partnerID=8YFLogxK

U2 - 10.1128/AAC.24.1.5

DO - 10.1128/AAC.24.1.5

M3 - Article

C2 - 6312874

AN - SCOPUS:0020639056

VL - 24

SP - 5

EP - 9

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 1

ER -