Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25

Nayna Nambiar; Shrea Tyagi; B.J. Reddig; P. Litt; D. Jaroni; Earl L. Blewett

Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25

Nayna Nambiar, Shrea Tyagi, B.J. Reddig, P. Litt, D. Jaroni, Earl L. Blewett

Biochemistry & Microbiology

Research output: Contribution to conference › Poster › peer-review

Abstract

Bacteriophage are viruses that infect, replicate and kill bacteria. Salmonella and EHEC food poisoning are caused by Salmonella and E. coli bacteria. Bacteriophage can be used to prevent food poisoning by application to food products or processing machinery. Bacteriophage J25 specifically infects Salmonella and E. coli bacteria. We cloned fragments of the J25 genome, sequence the DNA and used bioinformatics to identify J25. We used genome data from similar bacteriophage in Genbank to design primers to amplify the depolymerase-like gene. We amplified and cloned this gene. When expressed, the gene product will be test with bacteriophage food treatment where it should augment bacteriophage killing.

Original language	American English
State	Published - 4 Sep 2020
Event	Oklahoma State University Center for Health Sciences Research Day 2020 - Oklahoma State University Center for Health Sciences College of Osteopathic Medicine, Tulsa, United States Duration: 27 Feb 2020 → 28 Feb 2020

Conference

Conference	Oklahoma State University Center for Health Sciences Research Day 2020
Country/Territory	United States
City	Tulsa
Period	27/02/20 → 28/02/20

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https://hdl.handle.net/20.500.14446/324227

Cite this

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title = "Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25",

abstract = "Bacteriophage are viruses that infect, replicate and kill bacteria. Salmonella and EHEC food poisoning are caused by Salmonella and E. coli bacteria. Bacteriophage can be used to prevent food poisoning by application to food products or processing machinery. Bacteriophage J25 specifically infects Salmonella and E. coli bacteria. We cloned fragments of the J25 genome, sequence the DNA and used bioinformatics to identify J25. We used genome data from similar bacteriophage in Genbank to design primers to amplify the depolymerase-like gene. We amplified and cloned this gene. When expressed, the gene product will be test with bacteriophage food treatment where it should augment bacteriophage killing.",

author = "Nayna Nambiar and Shrea Tyagi and B.J. Reddig and P. Litt and D. Jaroni and Blewett, {Earl L.}",

year = "2020",

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day = "4",

language = "American English",

note = "Oklahoma State University Center for Health Sciences Research Day 2020 ; Conference date: 27-02-2020 Through 28-02-2020",

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TY - CONF

T1 - Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25

AU - Nambiar, Nayna

AU - Tyagi, Shrea

AU - Reddig, B.J.

AU - Litt, P.

AU - Jaroni, D.

AU - Blewett, Earl L.

PY - 2020/9/4

Y1 - 2020/9/4

N2 - Bacteriophage are viruses that infect, replicate and kill bacteria. Salmonella and EHEC food poisoning are caused by Salmonella and E. coli bacteria. Bacteriophage can be used to prevent food poisoning by application to food products or processing machinery. Bacteriophage J25 specifically infects Salmonella and E. coli bacteria. We cloned fragments of the J25 genome, sequence the DNA and used bioinformatics to identify J25. We used genome data from similar bacteriophage in Genbank to design primers to amplify the depolymerase-like gene. We amplified and cloned this gene. When expressed, the gene product will be test with bacteriophage food treatment where it should augment bacteriophage killing.

AB - Bacteriophage are viruses that infect, replicate and kill bacteria. Salmonella and EHEC food poisoning are caused by Salmonella and E. coli bacteria. Bacteriophage can be used to prevent food poisoning by application to food products or processing machinery. Bacteriophage J25 specifically infects Salmonella and E. coli bacteria. We cloned fragments of the J25 genome, sequence the DNA and used bioinformatics to identify J25. We used genome data from similar bacteriophage in Genbank to design primers to amplify the depolymerase-like gene. We amplified and cloned this gene. When expressed, the gene product will be test with bacteriophage food treatment where it should augment bacteriophage killing.

M3 - Poster

T2 - Oklahoma State University Center for Health Sciences Research Day 2020

Y2 - 27 February 2020 through 28 February 2020

ER -

Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25

Abstract

Conference

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