Bacteriophage are viruses that infect, replicate and kill bacteria. Salmonella and EHEC food poisoning are caused by Salmonella and E. coli bacteria. Bacteriophage can be used to prevent food poisoning by application to food products or processing machinery. Bacteriophage J25 specifically infects Salmonella and E. coli bacteria. We cloned fragments of the J25 genome, sequence the DNA and used bioinformatics to identify J25. We used genome data from similar bacteriophage in Genbank to design primers to amplify the depolymerase-like gene. We amplified and cloned this gene. When expressed, the gene product will be test with bacteriophage food treatment where it should augment bacteriophage killing.
|Original language||American English|
|State||Published - 4 Sep 2020|
|Event||Oklahoma State University Center for Health Sciences Research Day 2020 - Oklahoma State University Center for Health Sciences College of Osteopathic Medicine, Tulsa, United States|
Duration: 27 Feb 2020 → 28 Feb 2020
|Conference||Oklahoma State University Center for Health Sciences Research Day 2020|
|Period||27/02/20 → 28/02/20|
Nambiar, N., Tyagi, S., Reddig, B. J., Litt, P., Jaroni, D., & Blewett, E. L. (2020). Cloning and sequencing of the depolymerase-like gene from Bacteriophage J25. Poster session presented at Oklahoma State University Center for Health Sciences Research Day 2020, Tulsa, United States. https://shareok.org/handle/11244/324227