TY - JOUR
T1 - Characterization of virus-specific and cross-reactive monoclonal antibodies to Herpesvirus simiae (B virus)
AU - Blewett, Earl Linwood
AU - Black, Darla
AU - Eberle, R.
PY - 1996/11
Y1 - 1996/11
N2 - A panel of 13 monoclonal antibodies (MAbs) was produced that detect B virus (BV) proteins. Several of these MAbs were highly specific for BV, while the remainder cross-reacted in varying degrees with the other primate alphaherpesviruses. Utilizing western blot and radioimmunoprecipitation analysis, the MAbs were found to detect at least four distinct BV-infected cell antigens, several of which were composed of multiple polypeptides. One target antigen has been identified as the BV glycoprotein B (gB) homologue and was recognized by both virus-specific and cross-reactive MAbs. Although gB is an essential protein, none of the anti-gB MAbs neutralized infectious virus.
AB - A panel of 13 monoclonal antibodies (MAbs) was produced that detect B virus (BV) proteins. Several of these MAbs were highly specific for BV, while the remainder cross-reacted in varying degrees with the other primate alphaherpesviruses. Utilizing western blot and radioimmunoprecipitation analysis, the MAbs were found to detect at least four distinct BV-infected cell antigens, several of which were composed of multiple polypeptides. One target antigen has been identified as the BV glycoprotein B (gB) homologue and was recognized by both virus-specific and cross-reactive MAbs. Although gB is an essential protein, none of the anti-gB MAbs neutralized infectious virus.
UR - http://www.scopus.com/inward/record.url?scp=0029800928&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-77-11-2787
DO - 10.1099/0022-1317-77-11-2787
M3 - Article
C2 - 8922473
AN - SCOPUS:0029800928
SN - 0022-1317
VL - 77
SP - 2787
EP - 2793
JO - Journal of General Virology
JF - Journal of General Virology
IS - 11
ER -