Characterization of virus-specific and cross-reactive monoclonal antibodies to Herpesvirus simiae (B virus)

Earl Linwood Blewett, Darla Black, R. Eberle

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A panel of 13 monoclonal antibodies (MAbs) was produced that detect B virus (BV) proteins. Several of these MAbs were highly specific for BV, while the remainder cross-reacted in varying degrees with the other primate alphaherpesviruses. Utilizing western blot and radioimmunoprecipitation analysis, the MAbs were found to detect at least four distinct BV-infected cell antigens, several of which were composed of multiple polypeptides. One target antigen has been identified as the BV glycoprotein B (gB) homologue and was recognized by both virus-specific and cross-reactive MAbs. Although gB is an essential protein, none of the anti-gB MAbs neutralized infectious virus.

Original languageEnglish
Pages (from-to)2787-2793
Number of pages7
JournalJournal of General Virology
Volume77
Issue number11
DOIs
StatePublished - Nov 1996

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Cercopithecine Herpesvirus 1
Monoclonal Antibodies
Viruses
Glycoproteins
Radioimmunoprecipitation Assay
Antigens
Primates
Proteins
Western Blotting
Peptides

Cite this

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abstract = "A panel of 13 monoclonal antibodies (MAbs) was produced that detect B virus (BV) proteins. Several of these MAbs were highly specific for BV, while the remainder cross-reacted in varying degrees with the other primate alphaherpesviruses. Utilizing western blot and radioimmunoprecipitation analysis, the MAbs were found to detect at least four distinct BV-infected cell antigens, several of which were composed of multiple polypeptides. One target antigen has been identified as the BV glycoprotein B (gB) homologue and was recognized by both virus-specific and cross-reactive MAbs. Although gB is an essential protein, none of the anti-gB MAbs neutralized infectious virus.",
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Characterization of virus-specific and cross-reactive monoclonal antibodies to Herpesvirus simiae (B virus). / Blewett, Earl Linwood; Black, Darla; Eberle, R.

In: Journal of General Virology, Vol. 77, No. 11, 11.1996, p. 2787-2793.

Research output: Contribution to journalArticle

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