Characterization of a recombinant bacteriocin with potent activities against Clostridioides difficile

I-Hsiu Huang; Hsiang-Ning Chang; Yu-Jen Jiang; Yuan-Pin Hung; Jenn-Wei Chen; Joseph McCreary

Characterization of a recombinant bacteriocin with potent activities against Clostridioides difficile

I-Hsiu Huang, Hsiang-Ning Chang, Yu-Jen Jiang, Yuan-Pin Hung, Jenn-Wei Chen, Joseph McCreary

Research output: Contribution to conference › Poster › peer-review

Abstract

Clostridioides difficile is a spore-forming gram-positive bacteria and is the leading cause of antibiotic-associated diarrhea in hospitals worldwide. Immunocompromised conditions and disruptions of gut microbiota are risk factors for C. difficile infections (CDI). Currently no vaccines are available for the prevention of CDI and there are concerns for development of additional antibiotic resistance. In this study, we identified and characterized a bacteriocin from the probiotic Clostridium butyricum Miyari. Recombinant bacteriocin displayed a narrow spectrum of activity against only genus Clostridium and Clostridioides. Importantly, the recombinant bacteriocin displayed potent activity against C. difficile both in vitro and ex vivo. This study demonstrate the potential of developing alternative treatment strategies against CDI.

Original language	American English
Pages	52
State	Published - 22 Feb 2021
Event	Oklahoma State University Center for Health Sciences Research Days 2021: Poster presentation - Oklahoma State University Center for Health Sciences Campus, Tulsa, United States Duration: 22 Feb 2021 → 26 Feb 2021

Conference

Conference	Oklahoma State University Center for Health Sciences Research Days 2021
Country/Territory	United States
City	Tulsa
Period	22/02/21 → 26/02/21

Keywords

C. Difficile
Bacteriocin
Probiotic

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title = "Characterization of a recombinant bacteriocin with potent activities against Clostridioides difficile",

abstract = "Clostridioides difficile is a spore-forming gram-positive bacteria and is the leading cause of antibiotic-associated diarrhea in hospitals worldwide. Immunocompromised conditions and disruptions of gut microbiota are risk factors for C. difficile infections (CDI). Currently no vaccines are available for the prevention of CDI and there are concerns for development of additional antibiotic resistance. In this study, we identified and characterized a bacteriocin from the probiotic Clostridium butyricum Miyari. Recombinant bacteriocin displayed a narrow spectrum of activity against only genus Clostridium and Clostridioides. Importantly, the recombinant bacteriocin displayed potent activity against C. difficile both in vitro and ex vivo. This study demonstrate the potential of developing alternative treatment strategies against CDI. ",

keywords = "C. Difficile, Bacteriocin, Probiotic",

author = "I-Hsiu Huang and Hsiang-Ning Chang and Yu-Jen Jiang and Yuan-Pin Hung and Jenn-Wei Chen and Joseph McCreary",

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Characterization of a recombinant bacteriocin with potent activities against Clostridioides difficile. / Huang, I-Hsiu; Chang, Hsiang-Ning; Jiang, Yu-Jen et al.
2021. 52 Poster session presented at Oklahoma State University Center for Health Sciences Research Days 2021, Tulsa, Oklahoma, United States.

Research output: Contribution to conference › Poster › peer-review

TY - CONF

T1 - Characterization of a recombinant bacteriocin with potent activities against Clostridioides difficile

AU - Huang, I-Hsiu

AU - Chang, Hsiang-Ning

AU - Jiang, Yu-Jen

AU - Hung, Yuan-Pin

AU - Chen, Jenn-Wei

AU - McCreary, Joseph

PY - 2021/2/22

Y1 - 2021/2/22

N2 - Clostridioides difficile is a spore-forming gram-positive bacteria and is the leading cause of antibiotic-associated diarrhea in hospitals worldwide. Immunocompromised conditions and disruptions of gut microbiota are risk factors for C. difficile infections (CDI). Currently no vaccines are available for the prevention of CDI and there are concerns for development of additional antibiotic resistance. In this study, we identified and characterized a bacteriocin from the probiotic Clostridium butyricum Miyari. Recombinant bacteriocin displayed a narrow spectrum of activity against only genus Clostridium and Clostridioides. Importantly, the recombinant bacteriocin displayed potent activity against C. difficile both in vitro and ex vivo. This study demonstrate the potential of developing alternative treatment strategies against CDI.

AB - Clostridioides difficile is a spore-forming gram-positive bacteria and is the leading cause of antibiotic-associated diarrhea in hospitals worldwide. Immunocompromised conditions and disruptions of gut microbiota are risk factors for C. difficile infections (CDI). Currently no vaccines are available for the prevention of CDI and there are concerns for development of additional antibiotic resistance. In this study, we identified and characterized a bacteriocin from the probiotic Clostridium butyricum Miyari. Recombinant bacteriocin displayed a narrow spectrum of activity against only genus Clostridium and Clostridioides. Importantly, the recombinant bacteriocin displayed potent activity against C. difficile both in vitro and ex vivo. This study demonstrate the potential of developing alternative treatment strategies against CDI.

KW - C. Difficile

KW - Bacteriocin

KW - Probiotic

M3 - Poster

SP - 52

T2 - Oklahoma State University Center for Health Sciences Research Days 2021

Y2 - 22 February 2021 through 26 February 2021

ER -

Characterization of a recombinant bacteriocin with potent activities against Clostridioides difficile

Abstract

Conference

Keywords

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