CARE-2 fingerprinting of Candida albicans isolates.

Joachim Morschhäuser, Peter Staib, Gerwald Koehler

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The emergence of resistance to antifungal drugs in medically important fungi has become a significant problem in recent years. Probably the best-studied example is the development of resistance to the widely used antifungal agent fluconazole in the yeast Candida albicans. The availability of matched series of clinical isolates representing the same strain in which drug resistance developed over time has provided opportunities to detect cellular alterations that are correlated with drug resistance. We describe a method for DNA fingerprinting of C. albicans isolates based on Southern hybridization of genomic DNA with the C. albicans-specific repetitive DNA element CARE-2. Molecular typing with CARE-2 permits highly reliable discrimination of unrelated strains to ascertain that serial isolates recovered from individual patients indeed represent the same C. albicans strain.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalMethods in molecular medicine
Volume118
StatePublished - 1 Jan 2005

Fingerprint

Candida albicans
Drug Resistance
Fungal Drug Resistance
Nucleic Acid Hybridization
Molecular Typing
DNA Fingerprinting
Fluconazole
Antifungal Agents
DNA
Fungi
Yeasts

Cite this

Morschhäuser, Joachim ; Staib, Peter ; Koehler, Gerwald. / CARE-2 fingerprinting of Candida albicans isolates. In: Methods in molecular medicine. 2005 ; Vol. 118. pp. 27-34.
@article{7a85f7ad3fb04d28bcba07ba0f88378b,
title = "CARE-2 fingerprinting of Candida albicans isolates.",
abstract = "The emergence of resistance to antifungal drugs in medically important fungi has become a significant problem in recent years. Probably the best-studied example is the development of resistance to the widely used antifungal agent fluconazole in the yeast Candida albicans. The availability of matched series of clinical isolates representing the same strain in which drug resistance developed over time has provided opportunities to detect cellular alterations that are correlated with drug resistance. We describe a method for DNA fingerprinting of C. albicans isolates based on Southern hybridization of genomic DNA with the C. albicans-specific repetitive DNA element CARE-2. Molecular typing with CARE-2 permits highly reliable discrimination of unrelated strains to ascertain that serial isolates recovered from individual patients indeed represent the same C. albicans strain.",
author = "Joachim Morschh{\"a}user and Peter Staib and Gerwald Koehler",
year = "2005",
month = "1",
day = "1",
language = "English",
volume = "118",
pages = "27--34",
journal = "Methods in molecular medicine",
issn = "1543-1894",
publisher = "Humana Press",

}

CARE-2 fingerprinting of Candida albicans isolates. / Morschhäuser, Joachim; Staib, Peter; Koehler, Gerwald.

In: Methods in molecular medicine, Vol. 118, 01.01.2005, p. 27-34.

Research output: Contribution to journalArticle

TY - JOUR

T1 - CARE-2 fingerprinting of Candida albicans isolates.

AU - Morschhäuser, Joachim

AU - Staib, Peter

AU - Koehler, Gerwald

PY - 2005/1/1

Y1 - 2005/1/1

N2 - The emergence of resistance to antifungal drugs in medically important fungi has become a significant problem in recent years. Probably the best-studied example is the development of resistance to the widely used antifungal agent fluconazole in the yeast Candida albicans. The availability of matched series of clinical isolates representing the same strain in which drug resistance developed over time has provided opportunities to detect cellular alterations that are correlated with drug resistance. We describe a method for DNA fingerprinting of C. albicans isolates based on Southern hybridization of genomic DNA with the C. albicans-specific repetitive DNA element CARE-2. Molecular typing with CARE-2 permits highly reliable discrimination of unrelated strains to ascertain that serial isolates recovered from individual patients indeed represent the same C. albicans strain.

AB - The emergence of resistance to antifungal drugs in medically important fungi has become a significant problem in recent years. Probably the best-studied example is the development of resistance to the widely used antifungal agent fluconazole in the yeast Candida albicans. The availability of matched series of clinical isolates representing the same strain in which drug resistance developed over time has provided opportunities to detect cellular alterations that are correlated with drug resistance. We describe a method for DNA fingerprinting of C. albicans isolates based on Southern hybridization of genomic DNA with the C. albicans-specific repetitive DNA element CARE-2. Molecular typing with CARE-2 permits highly reliable discrimination of unrelated strains to ascertain that serial isolates recovered from individual patients indeed represent the same C. albicans strain.

UR - http://www.scopus.com/inward/record.url?scp=20344378468&partnerID=8YFLogxK

M3 - Article

C2 - 15888932

AN - SCOPUS:20344378468

VL - 118

SP - 27

EP - 34

JO - Methods in molecular medicine

JF - Methods in molecular medicine

SN - 1543-1894

ER -