Candida albicans mutant construction and characterization of selected virulence determinants

T. E. Motaung, J. Albertyn, C. H. Pohl, Gerwald Köhler

Research output: Contribution to journalReview articlepeer-review

7 Scopus citations


Candida albicans is a diploid, polymorphic yeast, associated with humans, where it mostly causes no harm. However, under certain conditions it can cause infections ranging from superficial to life threatening. This ability to become pathogenic is often linked to the immune status of the host as well as the expression of certain virulence factors by the yeast. Due to the importance of C. albicans as a pathogen, determination of the molecular mechanisms that allow this yeast to cause disease is important. These studies rely on the ability of researchers to create deletion mutants of specific genes in order to study their function. This article provides a critical review of the important techniques used to create deletion mutants in C. albicans and highlights how these deletion mutants can be used to determine the role of genes in the expression of virulence factors in vitro.

Original languageEnglish
Pages (from-to)153-165
Number of pages13
JournalJournal of Microbiological Methods
StatePublished - 2015


  • Candida albicans
  • Deletion mutant
  • Virulence factor


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