Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom

Rita de Cássia O. Collaço, Priscila Randazzo-Moura, Mariana L. Tamascia, Igor Rapp F. da Silva, Thalita Rocha, José C. Cogo, Stephen Hyslop, Charles G. Sanny, Léa Rodrigues-Simioni

Research output: Contribution to journalArticle

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Abstract

In this work, we examined some biochemical and biological activities of Bothrops fonsecai venom, a pitviper endemic to southeastern Brazil, and assessed their neutralization by commercial bothropic antivenom (CAv). Cross-reactivity of venom with CAv was also assessed by immunoblotting and size-exclusion high performance chromatography (SE-HPLC). Bothrops fonsecai venom had PLA2, proteolytic and esterase activities that were neutralized to varying extents by venom:antivenom ratios of 5:1 and 5:2 (PLA2 and esterase activities) or not significantly by either venom:antivenom ratio (proteolytic activity). The minimum hemorrhagic dose (69.2 μg) was totally neutralized by both ratios. Clotting time in rat citrated plasma was 33 ± 10.5 s (mean ± SD; n = 5) and was completely neutralized by a 5:2 ratio. Edema formation was dose-dependent (1–30 μg/site) and significantly inhibited by both ratios. Venom (10–300 μg/mL) caused neuromuscular blockade in extensor digitorum longus preparations; this blockade was inhibited best by a 5:2 ratio. Venom caused myonecrosis and creatine kinase release in vivo (gastrocnemius muscle) and in vitro (extensor digitorum longus) that was effectively neutralized by both venom:antivenom ratios. Immunoblotting showed that venom components of ~ 25–100 kDa interacted with CAv. SE-HPLC profiles for venom incubated with CAv or specific anti-B. fonsecai antivenom raised in rabbits (SAv) indicated that CAv had a higher binding capacity than SAv, whereas SAv had higher affinity than CAv. These findings indicate that B. fonsecai venom contains various activities that are neutralized to different extents by CAv and suggest that CAv could be used to treat envenoming by B. fonsecai.

Original languageEnglish
Pages (from-to)86-100
Number of pages15
JournalComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume191
DOIs
StatePublished - 1 Jan 2017

Fingerprint

Bothrops
Antivenins
Snake Venoms
Venoms
Esterases
Immunoblotting
High Pressure Liquid Chromatography
Neuromuscular Blockade
Creatine Kinase
Chromatography
Bioactivity
Brazil
Muscle
Rats
Edema

Keywords

  • Antivenom neutralization
  • Enzymatic activities
  • Local effects
  • Neuromuscular blockade
  • Venom:Antivenom binding

Cite this

Collaço, R. D. C. O., Randazzo-Moura, P., Tamascia, M. L., da Silva, I. R. F., Rocha, T., Cogo, J. C., ... Rodrigues-Simioni, L. (2017). Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom. Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 191, 86-100. https://doi.org/10.1016/j.cbpc.2016.08.008
Collaço, Rita de Cássia O. ; Randazzo-Moura, Priscila ; Tamascia, Mariana L. ; da Silva, Igor Rapp F. ; Rocha, Thalita ; Cogo, José C. ; Hyslop, Stephen ; Sanny, Charles G. ; Rodrigues-Simioni, Léa. / Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom. In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2017 ; Vol. 191. pp. 86-100.
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Collaço, RDCO, Randazzo-Moura, P, Tamascia, ML, da Silva, IRF, Rocha, T, Cogo, JC, Hyslop, S, Sanny, CG & Rodrigues-Simioni, L 2017, 'Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom', Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, vol. 191, pp. 86-100. https://doi.org/10.1016/j.cbpc.2016.08.008

Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom. / Collaço, Rita de Cássia O.; Randazzo-Moura, Priscila; Tamascia, Mariana L.; da Silva, Igor Rapp F.; Rocha, Thalita; Cogo, José C.; Hyslop, Stephen; Sanny, Charles G.; Rodrigues-Simioni, Léa.

In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, Vol. 191, 01.01.2017, p. 86-100.

Research output: Contribution to journalArticle

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T1 - Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom

AU - Collaço, Rita de Cássia O.

AU - Randazzo-Moura, Priscila

AU - Tamascia, Mariana L.

AU - da Silva, Igor Rapp F.

AU - Rocha, Thalita

AU - Cogo, José C.

AU - Hyslop, Stephen

AU - Sanny, Charles G.

AU - Rodrigues-Simioni, Léa

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N2 - In this work, we examined some biochemical and biological activities of Bothrops fonsecai venom, a pitviper endemic to southeastern Brazil, and assessed their neutralization by commercial bothropic antivenom (CAv). Cross-reactivity of venom with CAv was also assessed by immunoblotting and size-exclusion high performance chromatography (SE-HPLC). Bothrops fonsecai venom had PLA2, proteolytic and esterase activities that were neutralized to varying extents by venom:antivenom ratios of 5:1 and 5:2 (PLA2 and esterase activities) or not significantly by either venom:antivenom ratio (proteolytic activity). The minimum hemorrhagic dose (69.2 μg) was totally neutralized by both ratios. Clotting time in rat citrated plasma was 33 ± 10.5 s (mean ± SD; n = 5) and was completely neutralized by a 5:2 ratio. Edema formation was dose-dependent (1–30 μg/site) and significantly inhibited by both ratios. Venom (10–300 μg/mL) caused neuromuscular blockade in extensor digitorum longus preparations; this blockade was inhibited best by a 5:2 ratio. Venom caused myonecrosis and creatine kinase release in vivo (gastrocnemius muscle) and in vitro (extensor digitorum longus) that was effectively neutralized by both venom:antivenom ratios. Immunoblotting showed that venom components of ~ 25–100 kDa interacted with CAv. SE-HPLC profiles for venom incubated with CAv or specific anti-B. fonsecai antivenom raised in rabbits (SAv) indicated that CAv had a higher binding capacity than SAv, whereas SAv had higher affinity than CAv. These findings indicate that B. fonsecai venom contains various activities that are neutralized to different extents by CAv and suggest that CAv could be used to treat envenoming by B. fonsecai.

AB - In this work, we examined some biochemical and biological activities of Bothrops fonsecai venom, a pitviper endemic to southeastern Brazil, and assessed their neutralization by commercial bothropic antivenom (CAv). Cross-reactivity of venom with CAv was also assessed by immunoblotting and size-exclusion high performance chromatography (SE-HPLC). Bothrops fonsecai venom had PLA2, proteolytic and esterase activities that were neutralized to varying extents by venom:antivenom ratios of 5:1 and 5:2 (PLA2 and esterase activities) or not significantly by either venom:antivenom ratio (proteolytic activity). The minimum hemorrhagic dose (69.2 μg) was totally neutralized by both ratios. Clotting time in rat citrated plasma was 33 ± 10.5 s (mean ± SD; n = 5) and was completely neutralized by a 5:2 ratio. Edema formation was dose-dependent (1–30 μg/site) and significantly inhibited by both ratios. Venom (10–300 μg/mL) caused neuromuscular blockade in extensor digitorum longus preparations; this blockade was inhibited best by a 5:2 ratio. Venom caused myonecrosis and creatine kinase release in vivo (gastrocnemius muscle) and in vitro (extensor digitorum longus) that was effectively neutralized by both venom:antivenom ratios. Immunoblotting showed that venom components of ~ 25–100 kDa interacted with CAv. SE-HPLC profiles for venom incubated with CAv or specific anti-B. fonsecai antivenom raised in rabbits (SAv) indicated that CAv had a higher binding capacity than SAv, whereas SAv had higher affinity than CAv. These findings indicate that B. fonsecai venom contains various activities that are neutralized to different extents by CAv and suggest that CAv could be used to treat envenoming by B. fonsecai.

KW - Antivenom neutralization

KW - Enzymatic activities

KW - Local effects

KW - Neuromuscular blockade

KW - Venom:Antivenom binding

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