Abstract
Introduction/Objectives: As aging and menopause occur, a decrease in estrogen leads to a variety of physiologic changes. Bone maintenance is one of the major factors effected by decreased estrogen. Using an exploratory microarray analysis, we examined the arcuate nucleus (ARC) of the hypothalamus in estrogen-treated and oil-treated rats to identify differences in estrogen-related gene expression.
Methods: Six Sprague-Dawley rats underwent bilateral ovariectomies and were allowed a seven-day recovery period, after which three rats received oil injections, and three rats received estrogen injections. Biopsies were obtained from the ARC of the hypothalamus of each of the six rats and isolated ribonucleic acid (RNA) was sent to the Thermo Fischer Scientific-Microarray Research Service Lab for examination. Finally, we interpreted the results of the microarray analysis using the Transcriptome Analysis Console (TAC 4.0) software.
Results: Estrogen-treated rats displayed higher levels of parathyroid hormone-related protein (PTHrP) and S100 calcium-binding protein G compared to oil-treated rats.
Conclusions: Based on our exploratory microarray analysis, we determined that the ARC of the hypothalamus of estrogen-treated rats showed increased expression of PTHrP and S100 calcium-binding protein G, hormones related to bone health and bone maintenance. Keywords: arcuate nucleus, estrogen-treated, oil-treated
Methods: Six Sprague-Dawley rats underwent bilateral ovariectomies and were allowed a seven-day recovery period, after which three rats received oil injections, and three rats received estrogen injections. Biopsies were obtained from the ARC of the hypothalamus of each of the six rats and isolated ribonucleic acid (RNA) was sent to the Thermo Fischer Scientific-Microarray Research Service Lab for examination. Finally, we interpreted the results of the microarray analysis using the Transcriptome Analysis Console (TAC 4.0) software.
Results: Estrogen-treated rats displayed higher levels of parathyroid hormone-related protein (PTHrP) and S100 calcium-binding protein G compared to oil-treated rats.
Conclusions: Based on our exploratory microarray analysis, we determined that the ARC of the hypothalamus of estrogen-treated rats showed increased expression of PTHrP and S100 calcium-binding protein G, hormones related to bone health and bone maintenance. Keywords: arcuate nucleus, estrogen-treated, oil-treated
| Original language | American English |
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| State | Published - 14 Feb 2025 |
| Event | Oklahoma State University Center for Health Sciences Research Week 2025 - Oklahoma State University Center for Health Sciences, Tulsa, United States Duration: 10 Feb 2025 → 14 Feb 2025 https://medicine.okstate.edu/research/research_days.html |
Conference
| Conference | Oklahoma State University Center for Health Sciences Research Week 2025 |
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| Country/Territory | United States |
| City | Tulsa |
| Period | 10/02/25 → 14/02/25 |
| Internet address |
Keywords
- arcuate nucleus
- oil-treated
- estrogen-treated