Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain of Pasteurella multocida

R. S. Conrad, C. Galanos, Franklin Champlin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Lipopolysaccharides were extracted from freeze-dried cells of Pasteurella multocida strain P-1581 (serotype 8) by the phenol-chloroform-petroleum ether method and biochemically analysed using standard procedures. The primary neutral sugars were glucose, galactose and heptose. No deoxy sugars were detected. Amino sugars included galactosamine, glucosamine and glucosamine-6-phosphate. 3-Deoxy-D-manno-2-octulosonic acid was present at a relatively low concentration. The analyses included identification and quantification of phosphate and alanine. The primary fatty acids and their approximate relative ratios were 3-hydroxytetradecanoate and tetradecanoate 2:1. Tetradecanoic acid was bound almost exclusively by ester linkages. 3-Hydroxytetradecanoic acid was bound primarily by amide linkages, although significant numbers of ester-bound residues were noted. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses indicated that the lipopolysaccharides were of low molecular weight.

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalVeterinary Research Communications
Volume20
Issue number3
DOIs
StatePublished - 1 Jan 1996

Fingerprint

Pasteurella multocida
lipopolysaccharides
Lipopolysaccharides
Myristates
Deoxy Sugars
Esters
glucosamine
Heptoses
Amino Sugars
Galactosamine
linkage (genetics)
acids
Glucosamine
Myristic Acid
Chloroform
Phenol
esters
Galactose
Amides
Sodium Dodecyl Sulfate

Keywords

  • Cell membrane
  • Electrophoresis
  • Gas-liquid chromatography
  • Lipopolysaccharides
  • PAGE
  • Pasteurella multocida

Cite this

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abstract = "Lipopolysaccharides were extracted from freeze-dried cells of Pasteurella multocida strain P-1581 (serotype 8) by the phenol-chloroform-petroleum ether method and biochemically analysed using standard procedures. The primary neutral sugars were glucose, galactose and heptose. No deoxy sugars were detected. Amino sugars included galactosamine, glucosamine and glucosamine-6-phosphate. 3-Deoxy-D-manno-2-octulosonic acid was present at a relatively low concentration. The analyses included identification and quantification of phosphate and alanine. The primary fatty acids and their approximate relative ratios were 3-hydroxytetradecanoate and tetradecanoate 2:1. Tetradecanoic acid was bound almost exclusively by ester linkages. 3-Hydroxytetradecanoic acid was bound primarily by amide linkages, although significant numbers of ester-bound residues were noted. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses indicated that the lipopolysaccharides were of low molecular weight.",
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Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain of Pasteurella multocida. / Conrad, R. S.; Galanos, C.; Champlin, Franklin.

In: Veterinary Research Communications, Vol. 20, No. 3, 01.01.1996, p. 195-204.

Research output: Contribution to journalArticle

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AU - Galanos, C.

AU - Champlin, Franklin

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AB - Lipopolysaccharides were extracted from freeze-dried cells of Pasteurella multocida strain P-1581 (serotype 8) by the phenol-chloroform-petroleum ether method and biochemically analysed using standard procedures. The primary neutral sugars were glucose, galactose and heptose. No deoxy sugars were detected. Amino sugars included galactosamine, glucosamine and glucosamine-6-phosphate. 3-Deoxy-D-manno-2-octulosonic acid was present at a relatively low concentration. The analyses included identification and quantification of phosphate and alanine. The primary fatty acids and their approximate relative ratios were 3-hydroxytetradecanoate and tetradecanoate 2:1. Tetradecanoic acid was bound almost exclusively by ester linkages. 3-Hydroxytetradecanoic acid was bound primarily by amide linkages, although significant numbers of ester-bound residues were noted. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses indicated that the lipopolysaccharides were of low molecular weight.

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