Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain of Pasteurella multocida

R. S. Conrad, C. Galanos, F. R. Champlin

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Abstract

Lipopolysaccharides were extracted from freeze-dried cells of Pasteurella multocida strain P-1581 (serotype 8) by the phenol-chloroform-petroleum ether method and biochemically analysed using standard procedures. The primary neutral sugars were glucose, galactose and heptose. No deoxy sugars were detected. Amino sugars included galactosamine, glucosamine and glucosamine-6-phosphate. 3-Deoxy-D-manno-2-octulosonic acid was present at a relatively low concentration. The analyses included identification and quantification of phosphate and alanine. The primary fatty acids and their approximate relative ratios were 3-hydroxytetradecanoate and tetradecanoate 2:1. Tetradecanoic acid was bound almost exclusively by ester linkages. 3-Hydroxytetradecanoic acid was bound primarily by amide linkages, although significant numbers of ester-bound residues were noted. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses indicated that the lipopolysaccharides were of low molecular weight.

Original languageEnglish
Pages (from-to)195-204
Number of pages10
JournalVeterinary Research Communications
Volume20
Issue number3
DOIs
StatePublished - 1 Jan 1996

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Keywords

  • Cell membrane
  • Electrophoresis
  • Gas-liquid chromatography
  • Lipopolysaccharides
  • PAGE
  • Pasteurella multocida

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