Bacteriophage ΦX-174 as an aerobiological marker for surgical plume generated by the electromagnetic field focusing system

Joseph Price, W. Yamanashi, J. M.C. McGee

Research output: Contribution to journalArticle

Abstract

The aerosol of surgical plume could be measured effectively with the use of bacteriophage ΦX-174 as a biological marker, in contrast to previous methodologies reported by others. Recovery of virus plaqueforming units was highest from hydrophobic polytetrafluoro-ethylene membranes compared to hydrophobic polycarbonate screen filters or polyvinylidene difluoride depth filters, indicating that the method of virus recovery strongly affects the utility of a virus as an aerobiological marker. With this new method, surgical plume was indeed found in significant amounts when cutting tissue phantoms made with agar containing virus. The Electromagnetic Field Focusing System was used, which is a new thermal surgical device. The nominal power setting did not appear to be a factor in the amount of virus recovered. However, when pulse modulating the power by adjusting the crest factor from 1·4 to 4·3, a measure of the duty cycle for power delivery which adjusted the device from its cutting to haemostatic mode, a nearly five-fold increase in surgical plume, as evidenced by the recovery of ΦX-174 plaque forming units, was seen. The data indicate that bacteriophage ΦX-174 can be used effectively as an aerobiological marker for aerosols generated during clinical procedures, and reinforce the need to use a safety vacuum during aerosol generating procedures. The availability of a safe and economical biological marker for aerosols from clinical procedures, which may lead to acquired infections in hospital personnel, makes evaluation of procedures and containment systems markedly easier. The data also indicates that surgical plume biohazard may be present in other techniques that employ pulse modulation including surgical lasers and electrocauteries.

Original languageEnglish
Pages (from-to)39-50
Number of pages12
JournalJournal of Hospital Infection
Volume21
Issue number1
DOIs
StatePublished - 1 Jan 1992

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Electromagnetic Fields
Bacteriophages
Aerosols
Viruses
polycarbonate
Biomarkers
Hospital Personnel
Hazardous Substances
Equipment and Supplies
Electrocoagulation
Hemostatics
Vacuum
Agar
Lasers
Hot Temperature
Safety
Membranes
Infection

Keywords

  • Bacteriophage ΦX-174
  • Electromagnetic Field Focusing System
  • aerobiology
  • surgical plume hazard

Cite this

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abstract = "The aerosol of surgical plume could be measured effectively with the use of bacteriophage ΦX-174 as a biological marker, in contrast to previous methodologies reported by others. Recovery of virus plaqueforming units was highest from hydrophobic polytetrafluoro-ethylene membranes compared to hydrophobic polycarbonate screen filters or polyvinylidene difluoride depth filters, indicating that the method of virus recovery strongly affects the utility of a virus as an aerobiological marker. With this new method, surgical plume was indeed found in significant amounts when cutting tissue phantoms made with agar containing virus. The Electromagnetic Field Focusing System was used, which is a new thermal surgical device. The nominal power setting did not appear to be a factor in the amount of virus recovered. However, when pulse modulating the power by adjusting the crest factor from 1·4 to 4·3, a measure of the duty cycle for power delivery which adjusted the device from its cutting to haemostatic mode, a nearly five-fold increase in surgical plume, as evidenced by the recovery of ΦX-174 plaque forming units, was seen. The data indicate that bacteriophage ΦX-174 can be used effectively as an aerobiological marker for aerosols generated during clinical procedures, and reinforce the need to use a safety vacuum during aerosol generating procedures. The availability of a safe and economical biological marker for aerosols from clinical procedures, which may lead to acquired infections in hospital personnel, makes evaluation of procedures and containment systems markedly easier. The data also indicates that surgical plume biohazard may be present in other techniques that employ pulse modulation including surgical lasers and electrocauteries.",
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Bacteriophage ΦX-174 as an aerobiological marker for surgical plume generated by the electromagnetic field focusing system. / Price, Joseph; Yamanashi, W.; McGee, J. M.C.

In: Journal of Hospital Infection, Vol. 21, No. 1, 01.01.1992, p. 39-50.

Research output: Contribution to journalArticle

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