Atriopeptin Does Not Augment the Transvascular Flux of Macromolecules in the Hamster Cheek Pouch

Natriuretic peptides elaborated by atrial myocytes promote marked renal sodium and water excretion as a mechanism for fluid and electrolyte balance. Recent evidence suggests that atriopeptin (ANP) also targets the nonrenal vasculature as a site for enhanced fluid exchange. It remains unclear whether ANP alters microvascular integrity to facilitate the efflux of both plasma and proteins across the endothelial barrier, or if fluid exchange is selectively enhanced. This study evaluated the influence of ANP on macromolecular transport through the direct observation of microvessels in the hamster cheek pouch using fluorescent intravital microscopy. Fluorescein isothiocyanate conjugated to either bovine serum albumin or dextran 150,000 M _w was utilized as a permeability probe. Macromolecular efflux was quantified as fluorochrome clearance. The clearance of fluoresce in conjugted bovine serum albumin (57.94 ± 7.03) or fluorescein-conjugated dextran 150 (4.09 ± 1.35) remained unaltered by intravascular injection of 1 μg/kg ANP. Topical application of 40 ng to cheek pouch microvessels produced similar results. All pouches demonstrated positive leakage response to histamine 2.5 − 10⁶ M, increasing fluorochrome clearance approximately 2- to 11-fold. Bolus injection of 1 μg/kg ANP reduced mean arterial pressure, increased urine flow from 6.63 ± 2.59 μl/min to 8.20 ± 6.13 μl/min, and elevated sodium excretion from 1.37 ± 0.49 μEq/min to 2.54 ± 0.99 μEq/min. These results suggest that ANP fails to significantly alter the integrity of the protein-transporting channels in the microvascular exchange barrier.