Adaptive resistance to aminoglycosides antibiotics in Pseudomonas aeruginosa

L. B. Gilleland, H. E. Gilleland, J. A. Gibson, F. R. Champlin

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentration for the sensitive parent strain. These resistant strains exhibited characteristics previously associated with the impermeability type of resistance mechanism, i.e., they grew more slowly than the parent strain, the resistance was unstable in the absence of the antibiotic, and adaptation to one of the antibiotics conferred cross-resistance to other aminoglycosides. The adapted strains grew, with minimal morphological alterations, in concentrations of the various aminoglycosides that normally produced cell envelope damage, misshapen and filamentous cell formation, and cell lysis in the sensitive strain. Neither protein H1 nor phospholipid alterations appear to play a significant role in adaptive resistance to aminoglycoside antibiotics in this model system. The acquisition of adaptive resistance to the aminoglycoside antibiotics did not confer resistance to polymyxin B, another cationic antibiotic which is thought to share binding sites within the outer membrane with the aminoglycosides.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalJournal of Medical Microbiology
Volume29
Issue number1
DOIs
StatePublished - 1 Jan 1989

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Aminoglycosides
Pseudomonas aeruginosa
Anti-Bacterial Agents
Cell-in-Cell Formation
Polymyxin B
Tobramycin
Amikacin
Gentamicins
Phospholipids
Binding Sites
Membranes
Pharmaceutical Preparations
Proteins

Cite this

Gilleland, L. B. ; Gilleland, H. E. ; Gibson, J. A. ; Champlin, F. R. / Adaptive resistance to aminoglycosides antibiotics in Pseudomonas aeruginosa. In: Journal of Medical Microbiology. 1989 ; Vol. 29, No. 1. pp. 41-50.
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Adaptive resistance to aminoglycosides antibiotics in Pseudomonas aeruginosa. / Gilleland, L. B.; Gilleland, H. E.; Gibson, J. A.; Champlin, F. R.

In: Journal of Medical Microbiology, Vol. 29, No. 1, 01.01.1989, p. 41-50.

Research output: Contribution to journalArticle

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