Acetylcholine increases the level of diglyceride in mouse pancreas

M. W. Banschbach; R. L. Geison; Mabel Hokin-Neaverson

doi:10.1016/S0006-291X(74)80476-6

Acetylcholine increases the level of diglyceride in mouse pancreas

M. W. Banschbach
, R. L. Geison
, Mabel Hokin-Neaverson

Biochemistry & Microbiology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Incubation of mouse pancreas with 10^-5M or 10^-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10^-4M acetylcholine was blocked and reversed by 10^-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.

Original language	English
Pages (from-to)	714-718
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	58
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(74)80476-6
State	Published - 4 Jun 1974

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title = "Acetylcholine increases the level of diglyceride in mouse pancreas",

abstract = "Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.",

author = "Banschbach, \{M. W.\} and Geison, \{R. L.\} and Mabel Hokin-Neaverson",

note = "Funding Information: Acknow!edgements. This work was supported by Grants HD-131, HD-5342 and Grant NB-06745 from the National Institutes of Health, U.S. Public Health Service. The expert technical assistance of Mr. Ken Sadeghian is gratefully acknowledged.",

year = "1974",

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doi = "10.1016/S0006-291X(74)80476-6",

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T1 - Acetylcholine increases the level of diglyceride in mouse pancreas

AU - Banschbach, M. W.

AU - Geison, R. L.

AU - Hokin-Neaverson, Mabel

N1 - Funding Information: Acknow!edgements. This work was supported by Grants HD-131, HD-5342 and Grant NB-06745 from the National Institutes of Health, U.S. Public Health Service. The expert technical assistance of Mr. Ken Sadeghian is gratefully acknowledged.

PY - 1974/6/4

Y1 - 1974/6/4

N2 - Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.

AB - Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.

UR - https://www.scopus.com/pages/publications/0016249504

U2 - 10.1016/S0006-291X(74)80476-6

DO - 10.1016/S0006-291X(74)80476-6

M3 - Article

C2 - 4836273

AN - SCOPUS:0016249504

SN - 0006-291X

VL - 58

SP - 714

EP - 718

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Acetylcholine increases the level of diglyceride in mouse pancreas

Abstract

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