Acetylcholine increases the level of diglyceride in mouse pancreas

Martin Banschbach, R. L. Geison, Mabel Hokin-Neaverson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.

Original languageEnglish
Pages (from-to)714-718
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume58
Issue number3
DOIs
StatePublished - 4 Jun 1974

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Diglycerides
Acetylcholine
Pancreas
Tissue
Atropine
Phosphatidic Acids
Physostigmine
Phosphatidylinositols
Hydrolysis

Cite this

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abstract = "Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.",
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Acetylcholine increases the level of diglyceride in mouse pancreas. / Banschbach, Martin; Geison, R. L.; Hokin-Neaverson, Mabel.

In: Biochemical and Biophysical Research Communications, Vol. 58, No. 3, 04.06.1974, p. 714-718.

Research output: Contribution to journalArticle

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