Acetylcholine increases the level of diglyceride in mouse pancreas

M. W. Banschbach, R. L. Geison, Mabel Hokin-Neaverson

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Abstract

Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.

Original languageEnglish
Pages (from-to)714-718
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume58
Issue number3
DOIs
Publication statusPublished - 4 Jun 1974

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