TY - JOUR
T1 - Acetylcholine increases the level of diglyceride in mouse pancreas
AU - Banschbach, M. W.
AU - Geison, R. L.
AU - Hokin-Neaverson, Mabel
N1 - Funding Information:
Acknow!edgements. This work was supported by Grants HD-131, HD-5342 and Grant NB-06745 from the National Institutes of Health, U.S. Public Health Service. The expert technical assistance of Mr. Ken Sadeghian is gratefully acknowledged.
PY - 1974/6/4
Y1 - 1974/6/4
N2 - Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.
AB - Incubation of mouse pancreas with 10-5M or 10-4M acetylcholine (plus eserine) resulted in increased tissue diglyceride levels. Diglyceride rose from 0.5 μmoles per gram fresh tissue in unstimulated pancreas to 1.2 and 3.6 μmoles, respectively, in acetylcholine-stimulated tissue. The linear increase in diglyceride level with time at 10-4M acetylcholine was blocked and reversed by 10-5M atropine. When atropine was added, the level of diglyceride fell to the control, unstimulated value. The increase in diglyceride level was greater than could be accounted for by hydrolysis of phosphatidylinositol or phosphatidic acid.
UR - http://www.scopus.com/inward/record.url?scp=0016249504&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(74)80476-6
DO - 10.1016/S0006-291X(74)80476-6
M3 - Article
C2 - 4836273
AN - SCOPUS:0016249504
SN - 0006-291X
VL - 58
SP - 714
EP - 718
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -