A quantitative description of the Na-K-2Cl cotransporter and its conformity to experimental data

Bruce Benjamin, E. A. Johnson

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In epithelia, the Na-K-2Cl cotransporter cooperates with other transport mechanisms to produce transepithelial NaCl transport. The reaction cycle for the Na-K-2Cl cotransporter has been established experimentally, but whether it accounts, quantitatively, for experimental findings has yet to be established. The differential equations that describe the reaction cycle were formulated, and the steady-state solutions were obtained by digital computation. Conformity between this description and the experimental data obtained from the literature was explored by automatic searches for the sets of rate constants that yielded statistical best-fits to the experimental data. Fits were obtained from renal epithelial cell lines, HeLa cells, and duck erythrocytes. Results show that the reaction cycle for the Na-K-2Cl cotransporter conforms well, quantitatively, with the experimental data.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number3 42-3
StatePublished - 1 Sep 1997

Fingerprint

Ducks
HeLa Cells
Epithelium
Erythrocytes
Epithelial Cells
Kidney
Cell Line

Keywords

  • Cotransport
  • Epithelial cells
  • Erythrocytes
  • LLC-PK cell
  • MDCK cell
  • Shark rectal gland
  • Sodium chloride transport

Cite this

@article{731304c6313b4d54b575d7a903cfd369,
title = "A quantitative description of the Na-K-2Cl cotransporter and its conformity to experimental data",
abstract = "In epithelia, the Na-K-2Cl cotransporter cooperates with other transport mechanisms to produce transepithelial NaCl transport. The reaction cycle for the Na-K-2Cl cotransporter has been established experimentally, but whether it accounts, quantitatively, for experimental findings has yet to be established. The differential equations that describe the reaction cycle were formulated, and the steady-state solutions were obtained by digital computation. Conformity between this description and the experimental data obtained from the literature was explored by automatic searches for the sets of rate constants that yielded statistical best-fits to the experimental data. Fits were obtained from renal epithelial cell lines, HeLa cells, and duck erythrocytes. Results show that the reaction cycle for the Na-K-2Cl cotransporter conforms well, quantitatively, with the experimental data.",
keywords = "Cotransport, Epithelial cells, Erythrocytes, LLC-PK cell, MDCK cell, Shark rectal gland, Sodium chloride transport",
author = "Bruce Benjamin and Johnson, {E. A.}",
year = "1997",
month = "9",
day = "1",
language = "English",
volume = "273",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "3 42-3",

}

A quantitative description of the Na-K-2Cl cotransporter and its conformity to experimental data. / Benjamin, Bruce; Johnson, E. A.

In: American Journal of Physiology - Renal Physiology, Vol. 273, No. 3 42-3, 01.09.1997.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A quantitative description of the Na-K-2Cl cotransporter and its conformity to experimental data

AU - Benjamin, Bruce

AU - Johnson, E. A.

PY - 1997/9/1

Y1 - 1997/9/1

N2 - In epithelia, the Na-K-2Cl cotransporter cooperates with other transport mechanisms to produce transepithelial NaCl transport. The reaction cycle for the Na-K-2Cl cotransporter has been established experimentally, but whether it accounts, quantitatively, for experimental findings has yet to be established. The differential equations that describe the reaction cycle were formulated, and the steady-state solutions were obtained by digital computation. Conformity between this description and the experimental data obtained from the literature was explored by automatic searches for the sets of rate constants that yielded statistical best-fits to the experimental data. Fits were obtained from renal epithelial cell lines, HeLa cells, and duck erythrocytes. Results show that the reaction cycle for the Na-K-2Cl cotransporter conforms well, quantitatively, with the experimental data.

AB - In epithelia, the Na-K-2Cl cotransporter cooperates with other transport mechanisms to produce transepithelial NaCl transport. The reaction cycle for the Na-K-2Cl cotransporter has been established experimentally, but whether it accounts, quantitatively, for experimental findings has yet to be established. The differential equations that describe the reaction cycle were formulated, and the steady-state solutions were obtained by digital computation. Conformity between this description and the experimental data obtained from the literature was explored by automatic searches for the sets of rate constants that yielded statistical best-fits to the experimental data. Fits were obtained from renal epithelial cell lines, HeLa cells, and duck erythrocytes. Results show that the reaction cycle for the Na-K-2Cl cotransporter conforms well, quantitatively, with the experimental data.

KW - Cotransport

KW - Epithelial cells

KW - Erythrocytes

KW - LLC-PK cell

KW - MDCK cell

KW - Shark rectal gland

KW - Sodium chloride transport

UR - http://www.scopus.com/inward/record.url?scp=0030817711&partnerID=8YFLogxK

M3 - Article

C2 - 9321922

AN - SCOPUS:0030817711

VL - 273

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 3 42-3

ER -