TY - JOUR
T1 - A Pilot Analysis of Genome-Wide DNA Methylation Patterns in Mouse Cartilage Reveals Overlapping Epigenetic Signatures of Aging and Osteoarthritis
AU - Izda, Vladislav
AU - Dunn, Christopher M.
AU - Prinz, Emmaline
AU - Schlupp, Leoni
AU - Nguyen, Emily
AU - Sturdy, Cassandra
AU - Jeffries, Matlock A.
N1 - Funding Information:
This work was supported by NIH grants K08‐AR‐070891, P20‐GM‐125528, R61‐AR‐078075, R01‐AR‐076440, and Department of Defense CDMRP grant PR191652. The funding source was not involved in the writing of this article.
Publisher Copyright:
© 2022 The Authors. ACR Open Rheumatology published by Wiley Periodicals LLC on behalf of American College of Rheumatology.
PY - 2022/12
Y1 - 2022/12
N2 - Objective: Cartilage epigenetic changes are strongly associated with human osteoarthritis (OA). However, the influence of individual environmental OA risk factors on these epigenetic patterns has not been determined; herein we characterize cartilage DNA methylation patterns associated with aging and OA in a mouse model. Methods: Murine knee cartilage DNA was extracted from healthy young (16-week, n = 6), old (82-week, n = 6), and young 4-week post–destabilization of the medial meniscus (DMM) OA (n = 6) C57BL6/J mice. Genome-wide DNA methylation patterns were determined via Illumina BeadChip. Gene set enrichment analysis was performed by Ingenuity Pathway Analysis. The top seven most differentially methylated positions (DMPs) were confirmed by pyrosequencing in an independent animal set. Results were compared to previously published human OA methylation data. Results: Aging was associated with 20,940 DMPs, whereas OA was associated with 761 DMPs. Merging these two conditions revealed 279 shared DMPs. All demonstrated similar directionality and magnitude of change (Δβ 1.0% ± 0.2%, mean methylation change ± SEM). Shared DMPs were enriched in OA-associated pathways, including RhoA signaling (P = 1.57 × 10−4), protein kinase A signaling (P = 3.38 × 10−4), and NFAT signaling (P = 6.14 × 10−4). Upstream regulators, including TET3 (P = 6.15 × 10−4), immunoglobulin (P = 6.14 × 10−4), and TLR7 (P = 7.53 × 10−4), were also enriched. Pyrosequencing confirmed six of the seven top DMPs in an independent cohort. Conclusion: Aging and early OA following DMM surgery induce similar DNA methylation changes within a murine OA model, suggesting that aging may induce pro-OA epigenetic “poising” within articular cartilage. Future research should focus on confirming and expanding these findings to other environmental OA risk factors, including obesity, as well as determining late OA changes in mice.
AB - Objective: Cartilage epigenetic changes are strongly associated with human osteoarthritis (OA). However, the influence of individual environmental OA risk factors on these epigenetic patterns has not been determined; herein we characterize cartilage DNA methylation patterns associated with aging and OA in a mouse model. Methods: Murine knee cartilage DNA was extracted from healthy young (16-week, n = 6), old (82-week, n = 6), and young 4-week post–destabilization of the medial meniscus (DMM) OA (n = 6) C57BL6/J mice. Genome-wide DNA methylation patterns were determined via Illumina BeadChip. Gene set enrichment analysis was performed by Ingenuity Pathway Analysis. The top seven most differentially methylated positions (DMPs) were confirmed by pyrosequencing in an independent animal set. Results were compared to previously published human OA methylation data. Results: Aging was associated with 20,940 DMPs, whereas OA was associated with 761 DMPs. Merging these two conditions revealed 279 shared DMPs. All demonstrated similar directionality and magnitude of change (Δβ 1.0% ± 0.2%, mean methylation change ± SEM). Shared DMPs were enriched in OA-associated pathways, including RhoA signaling (P = 1.57 × 10−4), protein kinase A signaling (P = 3.38 × 10−4), and NFAT signaling (P = 6.14 × 10−4). Upstream regulators, including TET3 (P = 6.15 × 10−4), immunoglobulin (P = 6.14 × 10−4), and TLR7 (P = 7.53 × 10−4), were also enriched. Pyrosequencing confirmed six of the seven top DMPs in an independent cohort. Conclusion: Aging and early OA following DMM surgery induce similar DNA methylation changes within a murine OA model, suggesting that aging may induce pro-OA epigenetic “poising” within articular cartilage. Future research should focus on confirming and expanding these findings to other environmental OA risk factors, including obesity, as well as determining late OA changes in mice.
UR - http://www.scopus.com/inward/record.url?scp=85139952077&partnerID=8YFLogxK
U2 - 10.1002/acr2.11506
DO - 10.1002/acr2.11506
M3 - Article
AN - SCOPUS:85139952077
SN - 2578-5745
VL - 4
SP - 1004
EP - 1012
JO - ACR Open Rheumatology
JF - ACR Open Rheumatology
IS - 12
ER -