Identification and quantification of known or unknown proteins is essential in the biochemical and medical fields. Most importantly, the ability to elucidate a protein structure necessarily lends to an understanding of that protein’s function. This is because structure determines function. In this experiment, near infrared (NIR) spectroscopy was employed to support and confirm circular dichroism data for determining secondary structure of proteins – specifically the bovine IgG antibody. Due to the little time required to perform NIR analyses, this research has shown the potential for a new application of NIR in order to significantly reduce the elucidation time for a protein structure in the laboratory.
|Original language||American English|
|Number of pages||3|
|Journal||Journal of Undergraduate Chemistry Research|
|State||Published - 2021|