A Novel Protein Structure Elucidation Technique by Circular Dichroism and Near Infrared Spectroscopy

Stephen C Layng, Jackson Harris, Rebecca Miller, Maria Leising, Hannah Schroeder, Alex Nguyen, Meaghan Raab, Cole Phelps, Barry Hicks

Research output: Contribution to journalArticlepeer-review

Abstract

Identification and quantification of known or unknown proteins is essential in the biochemical and medical fields. Most importantly, the ability to elucidate a protein structure necessarily lends to an understanding of that protein’s function. This is because structure determines function. In this experiment, near infrared (NIR) spectroscopy was employed to support and confirm circular dichroism data for determining secondary structure of proteins – specifically the bovine IgG antibody. Due to the little time required to perform NIR analyses, this research has shown the potential for a new application of NIR in order to significantly reduce the elucidation time for a protein structure in the laboratory.
Original languageAmerican English
Pages (from-to)43-45
Number of pages3
JournalJournal of Undergraduate Chemistry Research
Volume20
Issue number3
StatePublished - 2021

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