A molecular genetic system for the pathogenic yeast Candida dubliniensis

Peter Staib, Sonja Michel, Gerwald Köhler, Joachim Morschhäuser

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Abstract

Candida dubliniensis is a recently described pathogenic yeast of the genus Candida that is closely related to Candida albicans but differs from it in several phenotypic and genotypic characteristics, including putative virulence traits, which may explain differences in the spectrum of diseases caused by the two species. In contrast to C. albicans, a molecular genetic system to study virulence of C. dubliniensis is lacking. We have developed a system for the genetic transformation of C. dubliniensis that is based on the use of the dominant selection marker MPA(R) from C. albicans that confers resistance to mycophenolic acid (MPA). Using this transformation system, a GFP (green fluorescent protein) reporter gene that was genetically engineered for functional expression in C. albicans and placed under control of the inducible C. albicans SAP2 (secreted aspartic proteinase) promoter was integrated into the C. dubliniensis genome. MPA-resistant transformants containing the SAP2P-GFP fusion fluoresced under SAP2-inducing conditions but not under SAP2-repressing conditions. These results demonstrate that the MPA(R) selection marker is useful for transformation of C. dubliniensis wild-type strains, that the GFP reporter gene is functionally expressed in C. dubliniensis, and that the C. albicans SAP2 promoter can be used for controlled gene expression in C. dubliniensis. These genetic tools will allow the dissection of the differences in virulence characteristics between the two pathogenic yeast species at the molecular level. (C) 2000 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalGene
Volume242
Issue number1-2
DOIs
Publication statusPublished - 25 Jan 2000

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Keywords

  • Controlled gene expression
  • GFP
  • Mycophenolic acid resistance
  • Reporter gene
  • Selection marker
  • Transformation

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