A Free Open-Source Method of Image Cytometry in Rat Dorsal Root Ganglia with Fluorescent Immunohistochemistry

Research output: Contribution to conferencePosterpeer-review

Abstract

Immunohistochemistry (IHC) is a valuable tool in clinical and biological research for evaluating proteins and other antigens in spatially bound tissue. In neuroinflammatory pain research, primary afferent neurons of the dorsal root ganglion (DRG) are studied to understand molecular signaling mechanisms involved in nociception (pain) and inflammation. Measuring IHC immunofluorescence in DRG neurons require manual hand tracing of nuclear and somatic boundaries, which is laborious, error-prone, and can require several weeks to collect the appropriate sample size with a mouse or pen-input display monitor. To overcome these limitations and increase standardization of sampling and measurement, we employed a reliable neuronal cytoplasmic reporter (NeuN), exclusive to DRG neuronal soma and nuclei, in a semi-automated algorithm-based approach of Image Cytometry in rat Dorsal Root Ganglion (ICDRGs).

Keywords: Image Cytometry, Primary Sensory Neurons, Automation Technique
Original languageAmerican English
Pagesp. 19
StatePublished - 22 Feb 2021
EventOklahoma State University Center for Health Sciences Research Days 2021: Poster presentation - Oklahoma State University Center for Health Sciences Campus, Tulsa, United States
Duration: 22 Feb 202126 Feb 2021

Conference

ConferenceOklahoma State University Center for Health Sciences Research Days 2021
Country/TerritoryUnited States
CityTulsa
Period22/02/2126/02/21

Keywords

  • Image Cytometry
  • Primary Sensory Neurons
  • Automation Technique

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