A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH

Joseph A. Price

doi:10.1016/j.jbbm.2006.10.008

A colorimetric assay for measuring phospholipase A₂ degradation of phosphatidylcholine at physiological pH

Joseph A. Price

Pathology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Phospholipase A₂ is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

Original language	English
Pages (from-to)	441-444
Number of pages	4
Journal	Journal of biochemical and biophysical methods
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/j.jbbm.2006.10.008
State	Published - 10 Apr 2007

Keywords

Bromothymol blue
High-throughput
Lipase
Microplate
PLA
Phospholipase

Access to Document

10.1016/j.jbbm.2006.10.008

Cite this

@article{ebb8ee7f0b5245cca63ad155874c4fc8,

title = "A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH",

abstract = "Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.",

keywords = "Bromothymol blue, High-throughput, Lipase, Microplate, PLA, Phospholipase",

author = "Price, \{Joseph A.\}",

year = "2007",

month = apr,

day = "10",

doi = "10.1016/j.jbbm.2006.10.008",

language = "English",

volume = "70",

pages = "441--444",

journal = "Journal of biochemical and biophysical methods",

issn = "0165-022X",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH

AU - Price, Joseph A.

PY - 2007/4/10

Y1 - 2007/4/10

N2 - Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

AB - Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

KW - Bromothymol blue

KW - High-throughput

KW - Lipase

KW - Microplate

KW - PLA

KW - Phospholipase

UR - https://www.scopus.com/pages/publications/33847643272

U2 - 10.1016/j.jbbm.2006.10.008

DO - 10.1016/j.jbbm.2006.10.008

M3 - Article

C2 - 17169434

AN - SCOPUS:33847643272

SN - 0165-022X

VL - 70

SP - 441

EP - 444

JO - Journal of biochemical and biophysical methods

JF - Journal of biochemical and biophysical methods

IS - 3

ER -

A colorimetric assay for measuring phospholipase A₂ degradation of phosphatidylcholine at physiological pH

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this