A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

Original languageEnglish
Pages (from-to)441-444
Number of pages4
JournalJournal of Biochemical and Biophysical Methods
Volume70
Issue number3
DOIs
StatePublished - 10 Apr 2007

Fingerprint

Bromthymol Blue
Phospholipases A2
Phosphatidylcholines
Assays
Pathology
Degradation
Enzymes
Substrates

Keywords

  • Bromothymol blue
  • High-throughput
  • Lipase
  • Microplate
  • PLA
  • Phospholipase

Cite this

@article{ebb8ee7f0b5245cca63ad155874c4fc8,
title = "A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH",
abstract = "Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.",
keywords = "Bromothymol blue, High-throughput, Lipase, Microplate, PLA, Phospholipase",
author = "Joseph Price",
year = "2007",
month = "4",
day = "10",
doi = "10.1016/j.jbbm.2006.10.008",
language = "English",
volume = "70",
pages = "441--444",
journal = "Journal of Biochemical and Biophysical Methods",
issn = "0165-022X",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH

AU - Price, Joseph

PY - 2007/4/10

Y1 - 2007/4/10

N2 - Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

AB - Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

KW - Bromothymol blue

KW - High-throughput

KW - Lipase

KW - Microplate

KW - PLA

KW - Phospholipase

UR - http://www.scopus.com/inward/record.url?scp=33847643272&partnerID=8YFLogxK

U2 - 10.1016/j.jbbm.2006.10.008

DO - 10.1016/j.jbbm.2006.10.008

M3 - Article

C2 - 17169434

AN - SCOPUS:33847643272

VL - 70

SP - 441

EP - 444

JO - Journal of Biochemical and Biophysical Methods

JF - Journal of Biochemical and Biophysical Methods

SN - 0165-022X

IS - 3

ER -