Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.
|Number of pages||4|
|Journal||Journal of Biochemical and Biophysical Methods|
|State||Published - 10 Apr 2007|
- Bromothymol blue