A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH

Joseph A. Price

doi:10.1016/j.jbbm.2006.10.008

A colorimetric assay for measuring phospholipase A₂ degradation of phosphatidylcholine at physiological pH

Joseph A. Price

Pathology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Phospholipase A₂ is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

Original language	English
Pages (from-to)	441-444
Number of pages	4
Journal	Journal of biochemical and biophysical methods
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/j.jbbm.2006.10.008
State	Published - 10 Apr 2007

Keywords

Bromothymol blue
High-throughput
Lipase
Microplate
PLA
Phospholipase

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10.1016/j.jbbm.2006.10.008

Cite this

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abstract = "Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.",

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AB - Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

KW - Bromothymol blue

KW - High-throughput

KW - Lipase

KW - Microplate

KW - PLA

KW - Phospholipase

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SN - 0165-022X

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JO - Journal of biochemical and biophysical methods

JF - Journal of biochemical and biophysical methods

IS - 3

ER -

A colorimetric assay for measuring phospholipase A₂ degradation of phosphatidylcholine at physiological pH

Abstract

Keywords

Access to Document

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