A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH

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Abstract

Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent substrate assays for it have been recently developed, there is a need for an assay with inexpensive commercially available substrates, useful when samples interfered with fluorescent assays, that is nonisotopic, continuous, conducted at physiological pH, and in a 96 well format. A reaction using bromothymol blue was developed that meets all these requirements.

Original languageEnglish
Pages (from-to)441-444
Number of pages4
JournalJournal of Biochemical and Biophysical Methods
Volume70
Issue number3
DOIs
StatePublished - 10 Apr 2007

Keywords

  • Bromothymol blue
  • High-throughput
  • Lipase
  • Microplate
  • PLA
  • Phospholipase

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