A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas

Lai Wa Tam, Nedra Wilson, Paul A. Lefebvre

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Little is known about how cells regulate the size of their organelles. In this study, we find that proper flagellar length control in Chlamydomonas reinhardtii requires the activity of a new member of the cyclin-dependent kinase (CDK) family, which is encoded by the LF2 (long flagella 2) gene. This novel CDK contains all of the important residues that are essential for kinase activity but lacks the cyclin-binding motif PSTAIRE. Analysis of genetic lesions in a series of lf2 mutant alleles and site-directed mutagenesis of LF2p reveals that improper flagellar length and defective flagellar assembly correlate with the extent of disruption of conserved kinase structures or residues by mutations. LF2p appears to interact with both LF1p and LF3p in the cytoplasm, as indicated by immunofluorescence localization, sucrose density gradients, cell fractionation, and yeast two-hybrid experiments. We propose that LF2p is the catalytic subunit of a regulatory kinase complex that controls flagellar length and flagellar assembly.

Original languageEnglish
Pages (from-to)819-829
Number of pages11
JournalJournal of Cell Biology
Volume176
Issue number6
DOIs
StatePublished - 12 Mar 2007

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Chlamydomonas
Flagella
Cyclin-Dependent Kinases
Phosphotransferases
Cell Fractionation
Chlamydomonas reinhardtii
Cyclins
Site-Directed Mutagenesis
Cell Size
Organelles
Fluorescent Antibody Technique
Sucrose
Catalytic Domain
Cytoplasm
Yeasts
Alleles
Mutation
Genes

Cite this

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A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas. / Tam, Lai Wa; Wilson, Nedra; Lefebvre, Paul A.

In: Journal of Cell Biology, Vol. 176, No. 6, 12.03.2007, p. 819-829.

Research output: Contribution to journalArticle

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