Abstract
Background: Sex-based differences are observed in chronic hepatitis C virus (HCV) infections leading to cirrhosis and hepatocellular carcinoma (HCC). We previously showed that liver estrogen receptor (ER)-mediated sex-based differences exist in cirrhosis and HCC. Liver ER-binding may lead to protective effects in pre-menopausal women. This study aimed to determine sex-based differential role of 17β-HSD13 in development of cirrhosis and HCC. We hypothesized that chronic HCV infection leads to dysregulated 17β-HSD13 in male cirrhosis and progression to HCC.
Methods: 65 (normal, cirrhosis, HCC) liver tissues were obtained from NIH Liver Tissue Bank. DIA proteomics mapped 4445 proteins, including 17β-HSD13. Clinical correlation with bilirubin, AST, ALP, and creatinine was determined(spearman’s). Immunohistochemistry validated 17β-HSD13 protein expression in tissues.
Results:17β-HSD13 had significantly lower expression in male cirrhosis group than females (P<0.05). In contrast, 17β-HSD13 expression in normal males was significantly greater than normal females (P<0.05). In HCC group, the expression in males was down-regulated compared to HCC females (P<0.05). Bilirubin values showed negative correlation with 17β-HSD13 expression (P<0.05) between cirrhosis and HCC (males alone and combined sex data).
Conclusions: Low 17β-HSD13 levels may predict worse disease in males with cirrhosis or HCC serving as disease biomarker. This novel report shows sex-based differences in 17β-HSD13 during HCV-induced cirrhosis development.
Methods: 65 (normal, cirrhosis, HCC) liver tissues were obtained from NIH Liver Tissue Bank. DIA proteomics mapped 4445 proteins, including 17β-HSD13. Clinical correlation with bilirubin, AST, ALP, and creatinine was determined(spearman’s). Immunohistochemistry validated 17β-HSD13 protein expression in tissues.
Results:17β-HSD13 had significantly lower expression in male cirrhosis group than females (P<0.05). In contrast, 17β-HSD13 expression in normal males was significantly greater than normal females (P<0.05). In HCC group, the expression in males was down-regulated compared to HCC females (P<0.05). Bilirubin values showed negative correlation with 17β-HSD13 expression (P<0.05) between cirrhosis and HCC (males alone and combined sex data).
Conclusions: Low 17β-HSD13 levels may predict worse disease in males with cirrhosis or HCC serving as disease biomarker. This novel report shows sex-based differences in 17β-HSD13 during HCV-induced cirrhosis development.
| Original language | American English |
|---|---|
| Pages | 35 |
| State | Published - 17 Feb 2023 |
| Event | Oklahoma State University Center for Health Sciences Research Week 2023 - Oklahoma State University Center for Health Sciences, 1111 W. 17th street, Tulsa, United States Duration: 13 Feb 2023 → 17 Feb 2023 https://medicine.okstate.edu/events/index.html?trumbaEmbed=view%3Devent%26eventid%3D160681489 |
Conference
| Conference | Oklahoma State University Center for Health Sciences Research Week 2023 |
|---|---|
| Country/Territory | United States |
| City | Tulsa |
| Period | 13/02/23 → 17/02/23 |
| Internet address |
Keywords
- 17β-HSD13
- Hepatitis C virus
- Liver cirrhosis